What is a high sucrose diet?
A high sucrose diet increased lipogenesis by repartitioning fatty acids to esterification away from fatty acid β-oxidation possibly by increasing malonyl-CoA, an inhibitor of carnitine palmitoyltransferase-1 (Roberts et al., 2008). From: Reference Module in Biomedical Sciences, 2014.
How do you make a high fat diet for mice?
High fat was prepared by mixing Indian vanaspati ghee and coconut oil in the ratio of 3:1 (v/v). It was given to the rats at a dose of 3 ml/kg body weight per day. High sugar diet consisted of 25% fructose which was orally fed to the rats.
Does sucrose cause weight gain?
Sucralose and weight gain Some studies have found no link between body weight and low-calorie sweeteners. Others have found that people who replace sugar with low-calorie sweeteners weigh ever-so-slightly less, on average — a difference, the researchers found, of fewer than two pounds.
How does sucrose affect metabolism?
There it may be converted into trioses that can be used for the de novo synthesis of triglycerides (TG) and cholesterol (Frayn and Kingman, 1995; Hellerstein, 2002). Thus, sucrose has the potential to influence both insulin sensitivity and lipid metabolism and there may be interconnectivity between the two.
What causes obesity in mice?
A problem with an immune system gene may lead to obesity and type 2 diabetes, a study done in mice suggests. Mice gained weight and developed health problems when they had a genetic defect that impairs some immune cells. The immune problems were linked to shifts in the gut microbiome.
Is sucrose good for weight loss?
Results showed that a high sucrose content in a hypoenergetic, low-fat diet did not adversely affect weight loss, metabolism, plasma lipids, or emotional affect.
What is the name of the gene that causes obesity in mice?
Perilipin-2 Null Mice are Protected Against Diet-Induced Obesity, Adipose Inflammation and Fatty Liver Disease. The Journal of Lipid Research, 2013; DOI: 10.1194/jlr.
What are the effects of excess fat and sucrose on C57BL mice?
In this study, we examined the effects in C57BL/6J mice of consuming excess fat or sucrose for a long period of time (55 wk). Another group of mice consumed a low-fat, low-sucrose (LL) diet. Mice fed the high-fat (HF) diet gained weight and developed hyperlipidemia and hyperleptinemia.
What type of diet do you use for production mice?
At JAX, we use several standard mouse diets. For most production colonies, we use a closed formula, custom diet upon the NIH-31, 6% fat (w/w) or 10% fat (w/w) diet (LabDiet ® formulations 5K52, 6%; 5K20, 11%). The diets for our production mice are available on the strain datasheets under “Technical Support/Dietary Information.”
What are high-fat high sucrose diets used for?
High-fat high sucrose diets are also used to induce aberrant blood glucose homeostasis in rodents ( Fernandes-Santos et al., 2009; Sampath and Karundevi, 2014 ). For example, a diet with addition of lard and sucrose can induce differences in weight in Sprague Dawley rats by day 30 ( Nakajima et al., 2015 ).
What is the difference between high fat and low sucrose diets?
Another group of mice consumed a low-fat, low-sucrose (LL) diet. Mice fed the high-fat (HF) diet gained weight and developed hyperlipidemia and hyperleptinemia. At 25 wk, but not at 55 wk, hepatic glucose-6-phosphatase (G6Pase) activity of the mice fed the high sucrose (HS) diet was greater than that of mice fed the LL or HF diet.