Is igepal the same as NP 40?
Nonidet P-40 and IGEPAL CA-630 are the same, those are merely different brand names. According to the manufacturer, Sigma-Aldrich, the two compounds are “chemically indistinguishable”. Both are octylphenoxy poly(ethyleneoxy)ethanol.
What does igepal stand for?
IGEPAL CA-630 is a nonionic, non-denaturing detergent. Its official IUPAC name is octylphenoxypolyethoxyethanol.
What is igepal co520?
IGEPAL CA-520 is a nonionic surfactant providing emulsification for non-polar hydrocarbon solvents and oils. Used in solvent emulsion cleaners, dry cleaning detergents, anti-icing additives for automotive gasoline, emulsifiers for pesticides and floor polishes.
Does NP-40 lysis nuclei?
NP-40 and Triton X-100 will not lyse nuclear membranes. After lysis, pellet the nuclei by centrifugation and transfer the supernatant to a new tube. If you wish to isolate both the nuclear and soluble fractions, resuspend the nuclear pellet in RIPA buffer.
What can I use instead of NP-40?
Usually NP-40 and triton are considered detergent of comparable potency. So if you wish to replace NP-40 by triton-X-100 and conserve similar conditions, you should use the same final concentration, e.g. 1% NP-40= 1% Triton-X-100.
Is NP-40 denatured?
Detergents come in varying strengths and are chosen based on whether extracted proteins need to be denatured or not. Sodium dodecyl sulfate is an ionic detergent that is strong enough to solubilize proteins and denature them. A weaker detergent such as NP-40 can be used to solubilize proteins but not denature them.
What does NP-40 stand for?
nonyl phenoxypolyethoxylethanol
NP-40 (also known as Tergitol-type NP-40 and nonyl phenoxypolyethoxylethanol) is a commercially available detergent with CAS Registry Number 9016-45-9. NP-40 is an ethoxylated nonylphenol for non-ionic surfactants and can act as emulsifier and demulsifier agent. NP-40. Names. Other names.
How do I make a NP-40 lysis buffer?
1. Prepare 1% NP40 lysis buffer (LB). (To make 50 ml of 1% NP40 lysis buffer, add 5 ml of 10% NP40, 500 μl of 1 mM EDTA, 1.5 ml of 150 mM NaCl, and 1 ml of 20 mM Tris–Cl, pH 7.4; and bring the total volume to 50 ml using dH2O.) 2.
What is the composition of IGEPAL ca 630?
IGEPAL CA-630. All IGEPAL CA surfactants are derived from octylphenol. This serves as the hydrophobic part. Different amounts of ethylene oxide are combined with this part to get a balance of hydrophobic/hydrophilic substances (measured by HLB ). This balance has an important impact on wetting detergency, foam, solubility, emulsification.
What is ca 630 used for?
IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.
What is the difference between IGEPAL CA-630 and Nonidet P-40?
Visit Product Comparison Guide IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40. Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.
Is ca 630 the same as P40?
IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40. Chemically indistinguishable from Nonidet P-40, which is no longer commercially available. May develop turbidity or sediment during storage.