How does Agilent 2100 Bioanalyzer work?
How does the Bioanalyzer work? The bioanalyzer instrument detects biomolecules by laser-induced fluorescence. Each LabChip® contains an interconnected set of microchannels that is used for separation of nucleic acid fragments based on their size as they are driven through it electrophoretically.
What is the purpose of a Bioanalyzer?
Applications. The Bioanalyzer can be used to assess the size and quality of DNA over a range of sizes and concentrations. The DNA 1000 assay can be used with extracted, clean DNA to look at the size profile of a sample from 25bp to 1000bp at a concentration of 0.1-50ng/ul.
How is Rin RNA calculated?
RIN is determined by the result of microcapillary electrophoretic RNA separation. Several features, including the total RNA ratio, the height of the 28S peak, the fast area ratio, and the marker height, are taken into consideration in the RIN algorithm.
What does a Bioanalyzer measure?
The Bioanalyzer can be used to assess the size and quality of DNA over a range of sizes and concentrations. The DNA 1000 assay can be used with extracted, clean DNA to look at the size profile of a sample from 25bp to 1000bp at a concentration of 0.1-50ng/ul.
How do I switch on the Agilent 2100 Bioanalyzer?
Switching on the Agilent 2100 Bioanalyzer Switching on the Agilent 2100 Bioanalyzer 1Make sure the 2100 Bioanalyzer instrument is connected to line power and connected to the PC. 2Turn on the line switch at the rear of the instrument. The status LED at the front of the 2100 Bioanalyzer instrument should light up.
What tests are included in the 2100 Bioanalyzer system (g2939ba)?
Test chips are required for the following tests and are included in the 2100 Bioanalyzer system (G2939BA). Test Chip Kit for Electrophoresis Methods (reorder no. G2938-68100) Diagnostics Test Purpose HV Stability and Accuracy TestTests high voltage accuracy and stability of all 16 high voltage power supplies and the high voltage controller.
What is the g2938a model used for?
The G2938A model performs diagnostic tests in combination with the electrode cartridge. Specific uses of the Agilent 2100 Bioanalyzer include DNA fragment analysis, protein applications, sampling QC in gene expression, and simple flow cytometric analysis of cells.
What is the Bioanalyzer firmware?
Firmware The firmware is a program to control the hardware components of the Agilent 2100 Bioanalyzer instrument. It is downloaded from your computer to the Agilent 2100 Bioanalyzer instrument and controls, among others, data transfer or the measurement procedures.