What are housekeeping genes for qPCR?
Housekeeping genes are cellular maintenance genes which regulate basic and ubiquitous cellular functions. In many RT-qPCR reactions, these genes are used as internal control genes without proper validation.
What is housekeeping cell?
Housekeeping genes are typically constitutive genes that are required for the maintenance of basal cellular functions that are essential for the existence of a cell, regardless of its specific role in the tissue or organism.
What are good housekeeping genes?
What is a Good Housekeeping Gene? The definition of a good housekeeping gene, is one that has stable expression in varying conditions, thus can be used to normalize other influences which may skew the result.
How many housekeeping genes are needed for qPCR?
Housekeeping genes encode proteins that are usually essential for the maintenance of cellular function and often remains constant under most experimental conditions. With this array, you can easily profile the expression of twelve (12) commonly used housekeeping genes in eight (8) samples using SYBR-green based qPCR.
What are the examples of housekeeping genes?
The proteins they code are generally involved in the basic functions necessary for the sustenance or maintenance of the cell. Examples of housekeeping genes include actin, GAPDH and ubiquitin.
Is cyclophilin A housekeeping gene?
The results suggest that the cyclophilin A gene is the most suitable housekeeping gene analysed for expression studies utilising uncultured bronchial airway epithelial cells from healthy and asthmatic children, and highlight the importance of validating housekeeping genes for each experimental model.
What is Cypa (Cyclophilin A)?
Cyclophilin A (CyPA) is a 20-kD chaperone protein secreted from VSMCs in response to ROS, and it stimulates VSMC proliferation and inflammatory cell migration in vitro and in vivo. The major topics that will be addressed in this review are a series of projects that were performed in our laboratory.
Is extracellular Cyclophilin A a mediator of neuroinflammation in amyotrophic lateral sclerosis?
Cyclophilin A, also known as peptidylprolyl cis-/trans-isomerase A (PPIA), as a foldase is beneficial intracellularly, but extracellularly has detrimental functions. We found that extracellular PPIA is a mediator of neuroinflammation in ALS.
How is Cyclophilin A secreted from smooth muscle?
Cyclophilin A is secreted by a vesicular pathway in vascular smooth muscle cells. Circ Res. 2006;98:811–817. [PubMed] [Google Scholar]
What is the function of human cyclophilin B?
Human cyclophilin B: A second cyclophilin gene encodes a peptidyl-prolyl isomerase with a signal sequence. Proc Natl Acad Sci USA. 1991;88:1903–1907. [PMC free article][PubMed] [Google Scholar] 22.