What is DharmaFECT?
DharmaFECT 1 is the most all-purpose transfection reagent, demonstrating efficient, low-toxicity delivery to over 80% of validated cell types.
How does plasmid transfection work?
By performing a process of DNA transfection, a plasmid which contains a gene of interest is efficiently delivered to the cells of interest. Upon delivery to the cells plasmid DNA reaches the nucleus during cell division, the gene of interest is transcribed and its transient expression is achieved.
What is the concentration of siRNA?
At what concentration should I make my siRNA stock solution? We recommend a concentration of 50 – 100 µM.
What is dharmafect 1?
Thermo Scientific DharmaFECT 1 is our most all-purpose transfection reagent formulation; demonstrating efficient, low-toxicity delivery to over 35 cell types.
What is dharmafect transfection reagent?
DharmaFECT transfection reagents provide efficient and reliable transfection at low siRNA/microRNA or plasmid reagent concentrations with minimal cellular toxicity. To attain efficient and reliable siRNA or microRNA transfection, we offer DharmaFECT 1-4 transfection reagents.
Which dharmafect reagent is best for my cell type?
Use the DharmaFECT Cell Type Guide to review validated formulations for common cell types. DharmaFECT kb is optimized to deliver plasmid DNA at low concentrations with minimal amount of transfection reagent and high cell viability. DharmaFECT Duo is the only optimized reagent to deliver both small RNA reagents with plasmid DNA.
How effective are dharmafect reagents for siRNA delivery?
Optimization studies with DharmaFECT 1, 2, 3, 4 and four other lipid transfection reagents were assessed for effective siRNA delivery (>80% knockdown) and low toxicity (>80% viability). Checkmarks indicate successful transfection.