What is acoustic flow cytometer?
Acoustic cytometry uses radiation pressure forces instead of or in addition to hydrodynamic focusing to position cells or particles in a flowing stream for analysis.
What is attune flow cytometer?
Attune Flow Cytometers are compact, benchtop cell analyzers that can be configured with up to four spatially separated lasers to flexibly design, run, and analyze panels of up to 14 colors.
What are the three major components of a flow cytometer?
The three main components of a flow cytometer are the fluidics, optics, and electronics (Figure 1). The fluidics system of a flow cytometer is responsible for transporting sample from the sample tube to the flow cell.
What is acoustic focusing?
The acoustic technology generates ultrasonic waves that act to transport particles to the center of the sample stream (acoustic focusing). The pre-focused stream is injected into the sheath stream, which applies hydrodynamic pressure to the sample (traditional hydrodynamic focusing).
What does attunement mean?
Attunement is the reactiveness we have to another person. It is the process by which we form relationships. Dr. Dan Siegel says, “When we attune with others we allow our own internal state to shift, to come to resonate with the inner world of another.
What is FACS sorting?
Fluorescence-activated cell sorting (FACS) is a technique to purify specific cell populations based on phenotypes detected by flow cytometry. This method enables researchers to better understand the characteristics of a single cell population without the influence of other cells.
What is a FACS plot?
–FACS is a process by which a sample mixture of cells is sorted according to their light scattering and fluorescence characteristics into two or more containers.
What is the process of hydrodynamic focusing?
Hydrodynamic focusing enables the flow channel to be coating by fluid, also referred to as sheath, creating a wall through which the sample can pass undisturbed. Essentially, a sandwich is created with two walls of sheath fluid and the sample core flowing on the inside.
What is the flow rate of the Attune™NXT acoustic focusing cytometer?
The Attune™NxT Acoustic Focusing Cytometer has a range of flow rates from 12.5 μL/minute to 1000 μL/minute. • At low sample rates (e.g., 12.5 μL/minute and 25 μL/minute), the instrument operates predominantly as a hydrodynamic focusing instrument.
Why does the Attune flow cytometers use pre-alignment?
The pre-alignment of cells allows the Attune Flow Cytometers to process samples quickly and acquire more statistically significant events even from very dilute samples. Acoustic focusing is doubly useful for cellular imaging on the Attune CytPix instrument, helping to position the cells precisely so that a sharp image is obtained.
What is the sample throughput rate of attune cytometric software?
The systems attain sample throughput rates of 1,000 µL/min, ten times faster than traditional cytometers without compromising data integrity. Attune Cytometric Software makes the system easy to use with an optional module for 21 CFR part 11 compliance.
How do I use the acoustic focusing cytometer safely?
For safety and biohazard guidelines, refer to the “Safety” appendix in the Attune®Acoustic Focusing Cytometer User Guide (PN 4453328). Wear appropriate protective eyewear, clothing, and gloves. Workflow Startup Run Daily Performance Test Collect Data Create Experiment Perform Auto- Compensation Shutdown Auto-Compensation