What is the composition of Lactophenol blue?
Lactophenol cotton blue (LCB) is a mixture of methyl blue, a histological stain, and lactophenol (a solution of phenol, lactic acid, and glycerol in water). It is used in wet-mount preparations for visualization of fungal structures, especially in medical mycology.
What are the components of Lactophenol?
The preparation has three components: phenol, which will kill any live organisms; lactic acid which preserves fungal structures, and cotton blue which stains the chitin in the fungal cell walls.
What can be identified with Lactophenol cotton blue staining?
Lactophenol Cotton Blue is useful in the recognition and presumptive identification of fungi. In tease mount, conidia or spores may be dislodged from the conidiogenous or sporogenous cells. Lactophenol without cotton blue is available and can be used for pigmented strains of molds.
What is the preservative used in Lactophenol cotton blue?
Lactic acid act as a preservative for fungi. The phenol portion kills the fungi. The cotton blue stains the fungal elements.
What are the four major components of Lactophenol Cotton Blue?
Reagents of Lactophenol Cotton Blue (LPCB) Staining Phenol Crystals (C6H5O4) 50g. Glycerol 100ml. Lactic acid (CH3CHOH COOH) 50ml. 70% ethanol.
Why is Lactophenol used for Molds?
Phenol kills fungus. Lactic acid acts as a clearing agent and helps preserve the fungal structures. Cotton blue is an aniline dye that stains the chitin in the fungal cell walls which adds colour to the fungal preparation thereby enhancing and contrasting the structures.
How do you make blue Lactophenol?
A preparation of 50ml Lactophenol cotton Blue staining solution is made up of:
- Distilled water 50ml.
- Cotton Blue (Aniline Blue) 0.125g.
- Phenol Crystals (C6H5O4) 50g.
- Glycerol 100ml.
- Lactic acid (CH3CHOH COOH) 50ml.
- 70% ethanol.
How does Lactophenol aniline blue work?
Remel Lactophenol Aniline Blue is a stain recommended for use in wet mount preparations for microscopic examination of fungi. Phenol precipitates cytoplasmic proteins and inactivates enzyme systems within the fungal cell. Glycerol prevents drying, allowing for examination of the stain 18-24 hours after preparation.
How do you use Lactophenol blue?
Procedure of Lactophenol Cotton Blue (LPCB) Staining Using a dropper or pipette, add one or two drops of Lactophenol Cotton Blue Solution (prepared above) before the ethanol dries off. Carefully cover the stain with a clean sterile coverslip without making air bubbles to the stain.
Why is Lactophenol used as a mounting medium?
What is the role of phenol in Lactophenol cotton blue solution?
Phenol: It acts as a disinfectant by killing any living organisms. Lactic acid: To preserve the fungal structures. Cotton blue: To stain or give color to the chitin on the fungal cell wall and other fungal structures.
Why is Lactophenol used as a mounting medium for molds?