Is GoTaq High-Fidelity?
GoTaq® Long PCR Master Mix | Long-Range PCR | High-Fidelity PCR | M4021 | Promega.
What is Q5 polymerase?
Q5® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification.
How do you choose Taq polymerase?
Guidelines to help you choose the right Taq formulation for your needs. Factors to consider when choosing a thermostable polymerase for your PCR include the intended application, enzyme characteristics, fidelity, reaction optimization needs and ease-of-use.
What is in GoTaq Green Master Mix?
Description: GoTaq® Green Master Mix(a) is a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.
Why is PQ polymerase better than Taq polymerase?
Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3′ to 5′ exonuclease proof reading activity, meaning that it works its way along the DNA from the 5′ endto the 3′ endand corrects nucleotidemisin corporation errors. Pfu DNA polymerase-generated PCRfragments will have fewer errors than Taq-generated PCR inserts.
Is Vent polymerase thermostable?
Vent® DNA Polymerase is a high-fidelity thermophilic DNA polymerase.
How fast does Q5 polymerase work?
Thermocycling Conditions for a Routine PCR:
| STEP | TEMP | TIME |
|---|---|---|
| Initial Denaturation | 98°C | 30 seconds |
| 25–35 Cycles | 98°C *50–72°C 72°C | 5–10 seconds 10–30 seconds 20–30 seconds/kb |
| Final Extension | 72°C | 2 minutes |
| Hold | 4–10°C |
What is GoTaq® DNA polymerase?
GoTaq® DNA Polymerase is a proprietary formulation of Taq polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq . The 5X GoTaq® Green and Colorless Reaction Buffers supplied with GoTaq® DNA Polymerase contain MgCl 2 at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction.
Why choose GoTaq® long PCR master mix?
GoTaq® Long PCR Master Mix is supplied with a control primer pair and human genomic DNA template to perform control reactions and test template quality. Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results.
What solutions does GoTaq® offer?
We offer numerous convenient solutions to meet your lab’s needs. GoTaq® G2 DNA Polymerase, the second generation of GoTaq® products, reliably amplifies a wide range of PCR templates and provides high-performing results due to improved manufacturing processes, increased reliability and consistency .
What is the concentration of MgCl2 in GoTaq G2 DNA polymerase?
GoTaq® G2 DNA Polymerase is supplied with 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction Buffer. Both buffers contain MgCl 2 at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction.