What is pBLU plasmid?
pBLU is a commercially produced bacterial plasmid that contains genes for ampicillin resistance (beta lactamase and beta galactosidase). It is often used in conjunction with an ampicillin-susceptible E. coli strain to teach students about transformation of eubacteria. It is 5,437 base pairs long.
What is the purpose of the LB amp ARA plate?
LB Agar Ampicillin-100, Plates has been used to plate and incubate the mixture of thawed bacteria and plasmid solution for vimentin expression. It has also been used in the generation of the Trypanosoma brucei RNAi library.
What does pUC18 stand for?
pUC18 is a commonly used plasmid cloning vector in E. The molecule is a double-stranded circular DNA (2686 base pairs in length).
What does pGLO stand for?
PGLO
| Acronym | Definition |
|---|---|
| PGLO | Persian Gay and Lesbian Organization (now Iranian Queer Organization) |
Where is pGLO from?
The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes, most notably the green fluorescent protein (GFP) and the ampicillin resistance gene. GFP was isolated from the jelly fish Aequorea victoria.
What is bacterial transformation in biology?
Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. It was first reported in Streptococcus pneumoniae by Griffith in 1928. DNA as the transforming principle was demonstrated by Avery et al in 1944.
Why does LB amp ARA glow?
LB/amp/ara (Luria Broth + ampicillin + arabinose): on which only transformed E coli grow. They do fluoresce as the arabinose in the medium causes the promoter to switch on the gene for GFP. The E coli starter culture and plasmid DNA have been freeze-dried. Both must be rehydrated.
What component of the LB amp ARA media is selective?
The component of the LB/AMP/ARA media that is selective is the AMP. Ampicillin added to the media only allows for the growth of organisms that have ampicillin resistance to grow.
Is pUC18 a plasmid?
Thermo Scientific pUC18 vector is a small, high copy number, E. coli plasmid, 2686 bp in length.
What is difference between pBR322 and pUC19?
The key difference between pBR322 and pUC19 is that pBR322 is a plasmid vector that is 4361 base pairs in length, while pUC19 is a plasmid vector that is 2686 base pairs in length. A cloning vector is a small piece of DNA that can be maintained in an organism stably.
Is pGLO a plasmid?
How were restriction enzymes used in pGLO?
Restriction digests also form part of the foundation for molecular biology in general. Recombinant DNA molecules (such as the pGLO plasmid) are traditionally made by cutting various desired DNA fragments with restriction enzymes, then ligating (joining) the pieces together.
What is a restriction map?
Introduction Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Exercise 1 Exercise 2 Reference Biol 390 F.S.C.\rBiology A restriction map is a diagram that indicates the relative positions of\rrestriction enzyme sites on a particular DNA sequence.
How many base pairs are there in pBLU?
A map of pBLU. pBLU is a commercially produced bacterial plasmid that contains genes for ampicillin resistance (beta lactamase and beta galactosidase). It is often used in conjunction with an ampicillin-susceptible E. coli strain to teach students about transformation of eubacteria. It is 5,437 base pairs long.
How many base pairs are in the ampicillin-susceptible plasmid pBLU?
pBLU is a commercially produced bacterial plasmid that contains genes for ampicillin resistance ( beta lactamase and beta galactosidase ). It is often used in conjunction with an ampicillin-susceptible E. coli strain to teach students about transformation of eubacteria. It is 5,437 base pairs long. There is a multiple cloning site in the lacZ gene.