What is F12 in DMEM?
DMEM/F-12 (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.
What is the difference between DMEM and DMEM F12?
DMEM and F12 are often mixed to combine the higher concentrations of components in DMEM with the wider range of Ham’s F12 ingredients. For instance, the addition of Ham’s F12 provides components such as biotin, putrescine, lipoic acid, proline, copper, and zinc that are not present in DMEM.
Does DMEM F12 have serum?
Together, it combines the high amount of glucose, amino acids and vitamins of DMEM, and the diversified components of F-12. It is a synthetic medium containing no serum or proteins and thus may require supplementation depending on the type of cell culture.
Does DMEM F12 have calcium?
The calcium concentration in Advanced DMEM/F12 is 1,05 mM).
What is in F12 media?
Nutrient Mixture F-12 was designed and developed specifically for the growth of Primary Rat Hepatocytes (PRHs) and Rat Prostate Epithelial Cells (RPECs). F-12 is based on Ham’s F-10 medium with increased concentrations of choline, inositol, putrescine and amino acids.
How long is DMEM F12 good for?
Finally, I know media (DMEM) by itself is generally stable for 12 months from manufacture when stored refrigerated, and sera is generally stable for 5 years when stored at -20°C or colder.
What is DMEM media used for?
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
What is Matrigel used for?
Matrigel, also termed Cultrex or EHS matrix, is prepared from an extract of Engelbreth-Holm-Swarm mouse tumors. It has been used as a model of basement membrane, which contacts with the basal layer of epithelial cells, endothelial cells, and fat and smooth muscle cells.
How much calcium is in DMEM?
Calcium concentrations of DMEM and RPMI-1640 media are 1.8 mM [8] and approximately 400 μM [9] respectively.
Does DMEM contain calcium?
The complete formulation is available. DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium….This DMEM is modified as follows:
| With | Without |
|---|---|
| • High Glucose | • L-glutamine |
| • Phenol Red | • Sodium Pyruvate |
| • HEPES | |
| • Calcium |
What is in DMEM media?
DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells.
Does DMEM expire?
What are the shelf-life and optimal storage conditions for cell culture media? Finally, I know media (DMEM) by itself is generally stable for 12 months from manufacture when stored refrigerated, and sera is generally stable for 5 years when stored at -20°C or colder.
What is DMEM F-12?
Gibco® DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12. This formulation combines DMEM’s high concentrations of glucose, amino acids and vitamins with F-12’s wide variety of components. For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
What supplements should I take with DMEM/F-12 media?
Advanced DMEM/F-12 requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media.
Where is advanced DMEM/F-12 manufactured?
For supply chain continuity, we manufacture Advanced DMEM/F-12 at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
What is the best cell culture media for Advanced DMEM/F-12?
Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat. The complete formulation is available.