What does it mean when a blood sample is lipemic?
Lipemia is defined as visible turbidity in serum or plasma samples due to the presence of lipoprotein particles, especially chylomicrons. The most common cause of turbidity is a high concentration of triglycerides [1,2].
How do you deal with a lipemic sample?
A recommended procedure for treating lipemic samples is centrifugation using ultracentrifuge which effectively removes lipids and allows measurement of large number of analytes (42,43). However, due to the high cost, this equipment it is not available in a large number of laboratories.
What causes lipemic sample?
The most common preanalytical cause of lipemic samples is inadequate time of blood sampling after the meal or parenteral administration of synthetic lipid emulsions.
Why is lipemic sample not accepted?
Second, lipemia can cause volume displacement, especially impacting analysis of electrolytes. Third, lipemic specimens can be nonhomogeneous, causing issues with how automated laboratory analyzers sense sample volume and pipette/aliquot specimens.
What causes too much fat in blood?
Most people have high levels of fat in their blood because they eat too much high-fat food. Some people have high fat levels because they have an inherited disorder. High lipid levels may also be caused by medical conditions such as diabetes, hypothyroidism, alcoholism, kidney disease, liver disease and stress.
How do you prevent lipemic blood?
Please avoid food with high fat content before blood donation. If a blood donor consumes food with high fat content such as oily foods which are fried or deep fried before blood donation it can lead to a transient rise in the triglycerides or cholesterol levels resulting in lipemic blood.
What is the normal appearance of serum after centrifugation?
After centrifugation, what remains is a clear, straw-colored liquid on top of a dark red clot (the clumped blood cells tangled in the fibrin mesh). This straw-colored, acellular liquid is called serum (see Figure 2). FIGURE 2: Serum – the acellular fraction of blood that has been allowed to clot.
Is lipemic serum acceptable for chemistry testing?
As a common interferer in clinical chemistry, lipemic specimens could be a source of significant analytical errors. Ultracentrifugation has been by far the only reliable, but an unavailable and expensive, method to eliminate the lipemic effect. Materials and Methods.
How do you remove serum lipemia?
Conclusions: High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples. LipoClear and 1,1,2-trichlorotrifluoroethane are unsuitable as they interfere with the measurement of certain parameters.
Does propofol cause lipemia?
Propofol is formulated in a lipid emulsion. Infusion of large doses of propofol over longer periods of time can result in lipemic blood (blood that appears milky because of high concentrations of triglyceride). Lipemia may also occur after a fatty meal.
What is the best drink to lower cholesterol?
Best drinks to improve cholesterol
- Green tea. Green tea contains catechins and other antioxidant compounds that seem to help lower “bad” LDL and total cholesterol levels.
- Soy milk. Soy is low in saturated fat.
- Oat drinks.
- Tomato juice.
- Berry smoothies.
- Drinks containing sterols and stanols.
- Cocoa drinks.
- Plant milk smoothies.
Do bananas help lower triglycerides?
The researchers also say that people with high triglycerides should focus on eating more vegetables; fruits that are lower in fructose such as cantaloupe, grapefruit, strawberries, bananas, peaches; high-fiber whole grains; and especially omega-3 fatty acids, which are found primarily in fatty fish such as salmon.
What is lipemic serum?
Lipemia or serum lipemic is the result of high concentrations of lipids in the blood. This causes turbidity or opacity of the blood serum due to the suspension of fatty particles in it; however, not all lipids produce turbidity of the serum.
What is the most common preanalytical cause of lipemic samples?
The most common preanalytical cause of lipemic samples is inadequate time of blood sampling after the meal or parenteral administration of synthetic lipid emulsions.
Is there any interference-free method for lipemic samples?
However, for lipemic samples, interference-free methods are not widely available, therefore interference of lipemia has to be quantified using different approach. This includes spiking of native sample with some interferent, in order to create lipemic sample.
How do you determine lipemia in chemistry?
Automated chemistry analyzers determine lipemia on the basis of the absorbance at specific wavelengths following dilution of the sample ( 1 ). False-positive results with this method are possible; in particular, the precipitation of paraproteins has been shown to correlate with high lipemic indices in clear samples ( 2 ).
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