What are C33a cells?
C-33 A [c-33a] are epithelial cells isolated from the cervix of a 66-year-old, White, uterine cancer patient. Expression of p53+ is elevated, and there is a point mutation at codon 273 resulting in a Arg -> Cys substitution.
What are SiHa cells?
SiHa is a cell line isolated from fragments of a primary uterine tissue sample from a 55-year-old, female, Japanese patient with squamous cell carcinoma. Genes expressed are p53+ and pRB+. This cell line is a suitable transfection host. Homo sapiens, human.
What is ATCC cell line?
ATCC or the American Type Culture Collection is a nonprofit organization which collects, stores, and distributes standard reference microorganisms, cell lines and other materials for research and development.
How many cell lines are maintained by ATCC?
ATCC has the world’s largest and most extensive product catalog of human and animal cell lines for research purposes. The cell biology collection includes more than 4,000 continuous cell lines available by species, tissue/disease types, and signaling pathways.
What is ATCC type strain?
A type strain may be provided to requestors from a stock initially propagated by the depositor or from the ATCC manufactured and authenticated inventory. Full inclusion as an ATCC General Collection strain will be dependent upon factors such as the need by the scientific community.
Why do we use ATCC strains?
That’s why ATCC scientists have collected, authenticated, and preserved microorganisms for nearly a century to ensure that researchers have access to the strains needed to develop vaccines and therapeutics, clean up oil spills, ensure healthier food and water, and develop products to protect our health and well-being.
How do you thaw cells in ATCC?
Thaw the vial by gentle agitation in a water bath at 37°C or the normal growth temperature for that cell line. Thawing should be rapid, approximately 2 minutes or until ice crystals have melted. 3. Remove the vial from the water bath and decontaminate it by dipping in or spraying with 70% ethanol.
How do you freeze cells in ATCC?
Freeze the cells gradually at a rate of -1°C/min until the temperature reaches -70°C to -80°C. The cells should not be left at -80°C for more than 24 to 48 hours. Once at -80°C, frozen cryovials should be transferred to the vapor phase of liquid nitrogen for long-term storage.
What is ATCC in microbiology?
ATCC is the premier source for microbial reference strains. Within our holdings are several ATCC special collections and projects representing critical areas of research.
How do you cite the ATCC?
Citation Data
- MLA. Jong, S. C. ATCC Names of Industrial Fungi. Rockville, Md. :American Type Culture Collection, 1994.
- APA. Jong, S. C. (1994). ATCC names of industrial fungi. Rockville, Md. :
- Chicago. Jong, S. C. ATCC Names of Industrial Fungi. Rockville, Md. :American Type Culture Collection, 1994.
How do you maintain ATCC strains?
Storage of slants is recommended at 2°C to 8°C for up to four weeks. The USP 36-NF 31 <51> recommends storage in liquid nitrogen or a mechanical freezer below −50°C. For long-term storage of frozen cultures, ATCC recommends the vapor phase of liquid nitrogen or a mechanical freezer at −80°C.
Why are cryoprotectants used for freezing?
Cryoprotectant agents are used to prevent ice formation, which causes freezing damage to the biological tissue when cooling the organs. They reduce the ice formation at any temperature by increasing the total concentration of all the solutes present in the system.