What color pigments are in spinach?
The green color of spinach is an indica- tion of the “freshness” of the product. The color of vegetables, for example spinach, is attributable the presence of various pigments, which primarily are the green chlorophylls and the yellow, orange, and red carotenoids.
What is the RF value of spinach?
Chlorophylls are the pigments primarily responsible for photosynthesis. They absorb red and blue light, and reflect green light, which is what gives leaves their green colour….Explanation.
| Pigment | Rf value |
|---|---|
| Chlorophyll a | 0.59 |
| Chlorophyll b | 0.42 |
| Anthocyanins | 0.32-0.62 |
| Xanthophylls | 0.15-0.35 |
What is the purpose of the spinach chromatography lab?
WAGHULDE.) Purpose: To separate plant pigments from spinach leaves using column chromatography. The leaves of plants contain a number of colored pigments generally falling into two categories, chlorophylls and carotenoids.
Why do spinach pigments separate in chromatography?
Pigments are separated according to differences in their relative solubilities. In order to extract these pigments from the thylakoid membranes of the chloroplasts, the organelles in which photosynthesis occurs, fresh, ground or torn leaves (preferably spinach) may be soaked in acetone or concentrated alcohol.
How many different pigments are contained in spinach?
Spinach leaves contain chlorophyll a and b and β-carotene as major pigments as well as smaller amounts of other pigments such as xanthophylls; these are oxidized versions of carotenes and phenophytins, which look like chlorophyll except that the magnesium ion (Mg+2) has been replaced by two hydrogen ions (H+).
Which pigment is the most soluble in spinach leaf?
The orange colored band, made of the pigment called carotenoids. is the most soluble in alcohol, so it traveled the farthest. The yellow xanthophylls are the next most soluble, followed by the blue-green chlorophyll A. The least soluble pigment is the yellow green chlorophyll B.
What is Rf value in chromatography?
In thin-layer chromatography, the retention factor (Rf) is used to compare and help identify compounds. The Rf value of a compound is equal to the distance traveled by the compound divided by the distance traveled by the solvent front (both measured from the origin).
How do you calculate Rf value in chromatography?
To calculate an Rf value, divide the distance travelled by the component – in other words, the distance from the starting pencil line to the coloured spot -by the distance travelled by the solvent. Rf values are important because each component has a fixed Rf value under a specific set of conditions.
How do you think the results would differ if you had used spinach leaves which had been stored in a dark room for five days before the experiment?
When plants are deprived of light, chlorophyll gradually breaks down and cannot be replenished. If the spinach leaves used for this lab had been stored in a dark room, there would have been less chlorophyll present in the extract and much lower peaks in the absorption spectrum would be expected.
Do your results confirm the initial hypothesis that the red pigment is hydrophobic?
Do results confirm that the red pigment is hydrophobic? No, the unknown pigment is water-soluble. Pigment is most likely attached to a protein which is water soluble and why we couldn’t extract it with hexane.
How do you think the results would differ if you had used spinach leaves which had been stored in a dark room for five days before the experiment explain your answer?
Why do spinach leaves appear green if yellow and orange pigments were present?
As I have noted in several previous essays, the yellow and orange colors in leaves are revealed when chlorophyll, the pigment responsible for making leaves appear green, is lost from the leaf. During the summer, these pigments were masked by the chlorophyll.
What are pigments found in spinach?
Spinach leaves contain chlorophyll a and b and β-carotene as major pigments as well as smaller amounts of other pigments such as xanthophylls; these are oxidized versions of carotenes and phenophytins, which look like chlorophyll except that the magnesium ion (Mg+2) has been replaced by two hydrogen ions (H+).
What are the principles of chromatography?
Principles of Chromatography. Chromatography is a separation method where the analyte is combined within a liquid or gaseous mobile phase., which is pumped through a stationary phase. Usually one phase is hydrophilic and the other lipophilic. The components of the analyte interact differently with these y=two phases.
What is column chromatography lab report?
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What is the purpose of chromatography?
Isolation,identification and analysis of different components of various mixtures.