What is Petroff method?
Decontamination by modified Petroff’s method is being practiced in many laboratories carrying out Mycobacterium tuberculosis culture and drug susceptibility testing. The method exposes mycobacteria to 4% sodium hydroxide for 30 min.
What is NALC NaOH method?
The chemical combination consisting of N-acetyl-l-cysteine–sodium hydroxide (NALC-NaOH) is primarily used for sputum decontamination for the isolation of Mycobacterium tuberculosis (12).
How do you collect sputum in mycobacterial culture?
To collect a sputum sample, you should do the following:
- Brush your teeth and rinse your mouth without using antiseptic mouthwash.
- Take a couple of long, deep breaths.
- Breathe deeply again and cough hard until sputum comes up.
- Spit out the sputum into the sample cup.
How does sputum smear microscopy work?
Smear microscopy involves collecting a biological sample (usually sputum or some other clinical material), fixing it thinly on a glass slide and then staining it with a dye that binds specifically to mycobacteria (making them easier to identify under a microscope).
What are the different digestion and decontamination method for Mycobacterium tuberculosis?
Decontamination and concentration of sputum specimens by modified Petroff’s method is one of the most commonly used methods for M. tuberculosis culture. N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) solution is recommended as a gentle but effective digesting and decontaminating agent.
Does tuberculosis need oxygen to survive?
It is a small bacillus that can withstand most disinfectants and often remains in its dormant state. To survive, it needs oxygen: it is thus called an aerobic bacterium. Mycobacterium tuberculosis grows slowly: it reproduces itself every 24 to 48 hours. This is extremely slow for bacteria.
How do you collect a sputum sample?
Take a very deep breath and hold the air for 5 seconds. Slowly breathe out. Take another deep breath and cough hard until some sputum comes up into your mouth. Spit the sputum into the plastic cup.
What is ABF test?
What is this test? An acid-fast bacteria (AFB) culture is done to find out if you have tuberculosis (TB) or another mycobacterial infection. Besides TB, the other main mycobacterial infections are leprosy and a TB-like disease that affects people with HIV/AIDS.
How do you collect a sputum sample for TB?
What does smear positive TB mean?
A case of pulmonary TB is considered to be smear-positive if one or more sputum smear specimens at the start of treatment are positive for AFB (provided that there is a functional EQA system with blind rechecking5).
How is Mycobacterium tuberculosis detected?
The Mantoux tuberculin skin test (TST) or the TB blood test can be used to test for M. tuberculosis infection. Additional tests are required to confirm TB disease. The Mantoux tuberculin skin test is performed by injecting a small amount of fluid called tuberculin into the skin in the lower part of the arm.
What kind of pathogen is Mycobacterium tuberculosis?
pathogenic bacteria
Mycobacterium tuberculosis (M. tb) is a species of pathogenic bacteria in the family Mycobacteriaceae and the causative agent of tuberculosis. First discovered in 1882 by Robert Koch, M. tuberculosis has an unusual, waxy coating on its cell surface primarily due to the presence of mycolic acid.