Category: Advices

What does the expression no man is an island actually mean?

no one is truly self-sufficient
The phrase no man is an island means that no one is truly self-sufficient, everyone must rely on the company and comfort of others in order to thrive.

Who said the quote No man is an island?

poet John Donne
English poet John Donne, writing in the 17th century, famously wrote that “no man is an island,” comparing people to countries, and arguing for the interconnectedness of all people with God.

What is the message of the poem no man is an island?

“No Man Is an Island” Themes Donne argues that every human being is connected to every other human being by comparing humanity itself to a vast landmass. No one is “an island” in the sense that no one is separate from this metaphorical “continent”; just by being human, everyone is part of humanity.

What is the poem talking about no man is an island?

In this poem, John Donne explores the idea of the connectedness of people. People are not isolated islands. We are all a part of a larger thing, and if one person dies, everyone is affected. Although this poem does not have a strong rhyme scheme, it is short and easy to memorize.

What is Donne saying the continent is?

No man is an island entire of itself; every man is a piece of the continent, a part of the main; if a clod be washed away by the sea, Europe is the less, as well as if a promontory were, as well as any manner of thy friends or of thine own were; any man’s death diminishes me, because I am involved in mankind.

What is the message of the song No man is an island?

The song was based on John Donne’s memorable quote “No man is an island.” The band’s drummer explains it like this: “This song is about how we are not meant to do life alone. So often we go through life and struggles, trials and difficult times, or even great times, and we try to do it by ourselves.

What is the message of John Donne No man is an island?

The main theme of this poem is community – that humans cannot live in isolation from each other. This is demonstrated by lines 1 and 3: the speaker says that no humans live separated from others (island), but all humans belong to one large collective (continent).

What is the reflection about the poem No man is an island?

In this poem the poets speak about humanity stating that a human being when isolated from others do not thrive. Once you decided living isolatedly for other person or group of person, you are definitely creating an island fir yourself. You’re trying to cut off everything and you probably get sick for being alone.

How do you know if you have chlamydia in your mouth?

Possible signs that you may have oral chlamydia include a sore throat that doesn’t go away, along with a low-grade fever; swollen lymph nodes; oral canker sores; or white spots in the back of the throat. In some cases, one might confuse these chlamydia symptoms with strep throat or some other kind of throat infection.

Does oral chlamydia go away?

Can you cure mouth chlamydia? Yes you can – chlamydia in the mouth, just as at other sites of the body, can be treated and completely removed with a simple course of antibiotics. However, it’s important to follow your treatment regime properly to ensure the infection is removed.

How long does chlamydia in the mouth take to show up?

Similar to other areas of the body, chlamydia symptoms may take about 7-21 days to show up in the throat. Chlamydia in the throat is typically caused by having oral sex with someone who has contracted the infection.

Does amoxicillin treat chlamydia?

The dosage that you will need will vary on a case-by-case basis. But in general, the Centers for Disease Control and Prevention (CDC) recommends taking 500 mg of amoxicillin orally three times per day for seven days to treat certain STDs, including chlamydia.

Does chlamydia live in saliva?

While kissing doesn’t transmit chlamydia, a number of other conditions can be transferred through kissing from saliva or open cuts around the mouth. Those conditions include: the common cold and other viral infections.

Is oral chlamydia common?

While chlamydia is among the most frequently reported bacterial STIs in the United States, chlamydia of the throat is uncommon. A person can develop it through oral contact with the penis of a person with chlamydia.

Can you get chlamydia from kissing?

Chlamydia isn’t spread through casual contact, so you CAN’T get chlamydia from sharing food or drinks, kissing, hugging, holding hands, coughing, sneezing, or sitting on the toilet. Using condoms and/or dental dams every time you have sex is the best way to help prevent chlamydia.

Can chlamydia affect you orally?

Although STIs primarily affect the genital areas, it’s possible that STIs like chlamydia can be spread via oral sex and cause throat problems. Doctors call chlamydia in the throat a pharyngeal chlamydia infection.

Can chlamydia live in your throat?

Oral chlamydia is caused by Chlamydia trachomatis bacteria and passed through oral sex. This is the same infection behind genital or anal chlamydia, which are more common. Symptoms include sore throat, fever, fatigue, mouth sores, and swollen tonsil or lymph nodes, but many cases show no symptoms at all.

Will 1000 mg of amoxicillin cure chlamydia?

Is 500mg of azithromycin enough to cure chlamydia?

A 500mg dose of azithromycin is not recommended by guidelines to cure chlamydia. There is also a chance it may increase the risk of C. trachomatis bacteria becoming resistant to it.

Is there a bridge over Lake Superior?

Bong Memorial Bridge, also known as the Bong Bridge, connects Duluth, Minnesota, and Superior, Wisconsin, via U.S. Highway 2 (US 2). Opened on October 25, 1985, it is roughly 11,800 feet (3,600 m) long, including about 8,300 feet (2,500 m) over water. It crosses the Saint Louis Bay, which drains into Lake Superior.

Can you walk across the lift bridge in Duluth?

Walk Across the Aerial Lift Bridge in Duluth Most people walk up to the bridge from below, watching the ships pass by. You can watch as it lifts vertically into the sky to a full height of 135 feet. What most people don’t know, is that you can actually walk across the bridge for a unique and exhilarating experience!

Why was the Duluth lift bridge built?

Reconstruction began in 1929. In order to ensure that tall ships could still pass under the bridge, the top span had to be raised to accommodate the new deck when raised. The support columns on either side were also modified so that they could hold new counterweights to balance the weight of the lifting portion.

When was the Duluth lift bridge built?

1929Aerial Lift Bridge / Construction started

How deep is the water in the Duluth Canal?

The project depths are 28 to 32 feet in the entrance; 27 feet deep in the iron-ore route channels, and 20 to 23 feet in the inner channels. There are two piers at the Duluth Entry and there are four piers at the Superior Entry.

Is the Blatnik Bridge in Duluth open?

While it was the last to close, the Minnesota Department of Transporation’s timeline for the project shows the connection from I-535 northbound to I-35 northbound is scheduled to be the first part of the reconstructed interchange to reopen in September 2022.

Who operates Duluth bridge?

Dave Campbell typically gets two questions when people learn he runs Duluth’s Aerial Lift Bridge.

Which crystal is best for chandelier?

Swarovski Crystal offers the finest chandelier crystal option available.

1. Swarovski Elements Crystal Glass.
2. Spectra Crystal.
3. Egyptian / Moroccan Crystal (Gemcut)
4. Turkish Crystal (Handcut, Heritage Handcut, Regal)
5. Italian Crystal (Legacy, Venetian)
6. K9 and Chinese Crystal.

What are the crystals called that hang from a chandelier?

What are the crystals hanging from a chandelier called? Chandelier crystals, which can be any color, cut and polished into many different sizes and shapes, are called pendalogues—occasionally spelled as pendeloques.

What is Czechoslovakian crystal?

Czech crystal is glass mass made from silica, soda and lead oxide. Czech glass is made from soda or potash, lime and silica and contains no lead oxide at all. Lead oxide makes the glass mass heavier, thicker and softer – which enables us to cut into it. It also increases the clarity and light refraction of glass mass.

What are the crystals on a chandelier made from?

The basics of chandelier crystals All crystals are made of glass. However, the glass used to craft chandelier crystals is very different from the glass in your windows, drinking cups, eyeglasses and so on. Crystal contains lead oxide, which gives it more weight and more refraction than normal glass.

How can you tell the difference between a glass chandelier and a crystal chandelier?

Generally, crystal chandeliers give off more color, but the fundamental difference between glass and crystal is the lead content. In the U.S., glass with 1 percent of lead or more is deemed a crystal, while in Europe glass becomes a crystal only when lead content exceeds 10 percent.

How can you tell if you have a Swarovski crystal chandelier?

Check for laser logo: If you are purchasing Swarovski Strass Crystal it is easy to tell if it is authentic as there is a laser etched trapezoid logo inside the crystal. If the crystal is the highest quality Swarovski, vendors will always state that it is Swarovski Strass.

What do chandeliers symbolize?

Since chandeliers production began in the world, this most luxurious of all decorative items was set to be a symbol of achievement, wealth and high societal status. The word ‘chandelier’ derived from the Old French word chandelier, itself a variation from the Latin candelabrum which denotes candle holder.

What are the things that hang from chandeliers?

What are chandelier pieces called?

• Finials.
• Canopies/ Collars.
• Scroll.
• Body Dish.
• Chains.
• Fount.
• Candle.
• Bobeche.

How can you tell a Czech crystal?

Identification can be hard, as most glass is unmarked and labels were usually washed off. The way to get your eye in is to look through specialist books. Colour can be a clue: amber-brown and green is a hallmark combination.

Is Bohemian crystal real crystal?

The difference between glass and crystal In the western world, however, the crystal is most often referred to as glass with the presence of lead. What we at Wranovsky talk about when we say Bohemian crystal is glass manufactured in the traditional Bohemian region with at least 24% content of lead.

How do I know if my chandelier is crystal?

The crystal shines at first glance, it is shiny, and a wide range of colours can be seen through the light passing through it. The glass does not shine and does not reflect the light rays into colour combinations. Crystal can be processed better, its edges are very fine, thin.

How do you clean old crystal chandeliers?

Prepare a cleaning solution of one part isopropyl alcohol to four parts distilled water in a spray bottle. Spray a small amount of the solution on a white cotton glove or lint free cloth. Wipe the crystal or glass with the damp cloth, and then dry it immediately with another glove or cloth.

Which Chandeliers have the best quality?

All our chandeliers have the best quality – all parts are from crystal and metal ( polished brass – gold color, polished nickel – silver color or antique brass) and are made in the Czech Republic (Bohemia, Europe Union). At all our chandeliers you can use LED lights, halogen lights or classic lights. We offer our chandeliers with

Can you deliver crystal chandeliers and lamps anywhere in the world?

We will send you the ordered crystal chandeliers and lamps anywhere in the world. Safely and reliably. Terms and prices of delivery of goods. Chandeliers according to your wishes.

What kind of Lights do you use in your chandeliers?

At all our chandeliers you can use LED lights, halogen lights or classic lights. We offer our chandeliers with SPECTRA ® Swarovski ® Crystal (Leadfree Crystal), Full Lead Bohemia Crystal ® or Strass ® Swarovski ® Crystal!!

What is inside pan Mee?

Pan Mee (板面) is a popular Hakka noodle dish in Malaysia, especially in central and southern part of Malaysia where most Hakka people reside. Hakka Pan Mee is made with a simple flour-based dough, with anchovy broth, and topped with crispy fried anchovies, ground pork, shiitake mushrooms, and some vegetables.

How to cook egg lo mein noodles?

All egg noodles should be par-boiled in water before stir-frying (though be careful, some brands sell their noodles pre-boiled and ready-to-fry). Lo mein noodles take about 3 minutes. After they’re cooked, I briefly run them under cold water to chill them rapidly.

What is Pan Mee English?

Banmian (simplified Chinese: 板面; traditional Chinese: 板麵; pinyin: bǎnmiàn) or pan mee (Min Nan Chinese: pán-mī) is a popular Chinese noodle dish, consisting of handmade noodles served in soup.

How do you pronounce Pan Mee?

“Pan mee (Chinese: 板麺, pronounced as ban mian) is a Hakka-style noodle, originating from Malaysia.

Do you boil lo mein noodles?

Lo Mein Vs Chow Mein A traditional Chow Mein has boiled noodles that are stir-fried until slightly crisped, while Lo Mein is boiled then tossed in a sauce without cooking the noodles in the Wok.

Where did pan mee originate from?

China
Singapore
Banmian/Origins

How to make pan mee?

Pan Mee – Malaysian dish with hand-torn noodles in a rich anchovy broth and topped with ground pork, veggie and crispy anchovies, Wash the two cups of anchovies thoroughly with water. Rinse about 5 times or until the water turns clear. Drain and set aside. Wash the pork bones thoroughly and scald them with some hot boiling water.

What is Moo goo gai pan supposed to taste like?

This is exactly what Moo Goo Gai Pan is supposed to taste like. It’s not meant to be spicy but that’s probably what’s “lacking” for some of us. I didn’t have water chestnuts so I peeled and sliced broccoli stems instead. That added the crunch and I did add some heat but only AFTER tasting the original recipe.

What is the best way to cook Moo-goo?

Most Chinese restaurants use this technique for their Moo-Goo’s & other meat dishes. 1 Egg white & 1 TBsp corn starch mixed into a slurry. Add meat and refrigerate 1/2 hour – then cook as instructed.

What is Panpan Mee?

Pan Mee (板面) is a popular Hakka noodle dish in Malaysia, especially in central and southern part of Malaysia where most Hakka people reside.

What is a good saying when someone dies?

The best things to say when someone dies

• I’m so sorry for your loss.
• You are in our thoughts and prayers.
• They will be so missed.
• I’m very sorry to hear this tragic news.
• I’m shocked and saddened by this devastating news.
• I can’t imagine how you must feel right now.
• If you want to talk, I’m here at any time.

How do you honor someone who is dying?

The 8 best ways to honor a loved one who has passed

1. Experience their favorite things.
2. Make a donation.
3. Create a memorial.
4. Create a grant or scholarship.
5. Complete their unfinished projects.
6. Keep something of theirs close by.
7. Light a candle.
8. Establish a celebration of their life.

How do you say goodbye at a funeral?

How to Say Goodbye to the Deceased If You Can Attend the Funeral

1. Attend the viewing. One of the last opportunities you get to see your loved one may be at the viewing.
2. Donate toward final expenses.
3. Plan a memorial.
4. Write a short eulogy.
5. Sing a song.
6. Send a gift.
7. Light a candle.
8. Attend by video.

What do dying patients want?

Generally speaking, people who are dying need care in four areas: physical comfort, mental and emotional needs, spiritual needs, and practical tasks. Of course, the family of the dying person needs support as well, with practical tasks and emotional distress.

How do you say goodbye to a dying loved one?

Some of the most common are:

1. “I love you.” These three words are one of the greatest phrases to use in goodbyes.
2. “I forgive you.” or “I’m sorry.” These are powerful goodbye words and can transform you and the person who receives them for a lifetime.
3. “Thank you” is another comforting goodbye phrase.

What are some inspirational poems on death?

Life Is But A Dream. For death has just released me,yet in your dream you weep.

In Our Hearts. But that is nothing new. And days before that,too.

My Angel. Hey,I am 14 years old. I also lost my dad.

Letter From Heaven. I hope this doesn’t reach you too late. Because life up here is great.

Watch The Rain. Watch the rain.

What are some famous poems about missing someone?

The Wind is Blowin’ – Charles Badger Clark

When You Go – Jessie Belle Rittenhouse

How It Used To Be – Melanie Edwards

Love – Pablo Neruda

Every Moment

Heart Song

How to tell if a loved one is dying?

Not want food or drink

Stop peeing and having bowel movements

Grimace,groan,or scowl from pain

What to do upon the death of a loved one?

Call your banks and credit unions to update the account holder’s information.

Contact your financial advisor to change your beneficiary information and assign assets according to the will or trust.

Contact your insurance policy providers including those for life insurance and car insurance.

What do you call a 20 sided shape?

In geometry, an icosagon or 20-gon is a twenty-sided polygon. The sum of any icosagon’s interior angles is 3240 degrees.

What 3D shape has 21 faces?

The eight convex deltahedra

Regular deltahedra
Name	Faces	Edges
snub disphenoid	12	18
triaugmented triangular prism	14	21
gyroelongated square bipyramid	16	24

What is a shape with 20 faces 12 vertices and 30 edges?

The 20 faces of the icosahedron are equilateral triangles; they meet in 30 edges and 12 vertices. The dodecahedron consists of 12 faces that are regular pentagons, and comprises 30 edges and 20 vertices.

What 3D shape has 24 faces?

Rhombicuboctahedron

Rhombicuboctahedron
Type	Archimedean solid Uniform polyhedron
Elements	F = 26, E = 48, V = 24 (χ = 2)
Faces by sides	8{3}+(6+12){4}
Conway notation	eC or aaC aaaT

Is a dodecahedron a prism?

It is one of 18 convex uniform polyhedral prisms created by using uniform prisms to connect pairs of parallel Platonic solids or Archimedean solids.

Is a dodecahedron a Platonic solid?

Platonic solid, any of the five geometric solids whose faces are all identical, regular polygons meeting at the same three-dimensional angles. Also known as the five regular polyhedra, they consist of the tetrahedron (or pyramid), cube, octahedron, dodecahedron, and icosahedron.

What 3D shape has exactly 4 faces?

There are 4 faces, 6 edges and 4 vertices in a regular tetrahedron. Which shape has 4 faces 6 edges and 4 vertices? Tetrahedron

What 3D shapes that have at least 1 square face?

In the case of 3D hexagons, the hexagonal base is usually a regular hexagon. For example, a truncated octahedron can be considered a 3D Hexagon because it has a hexagonal base. Which shape can have a square face in math? The faces of a cuboid are either rectangular or square. Thus, a cuboid has all 6 faces flat. All the faces of a cube are flat.

What kind of 3D shape has 17 faces?

If you look at this shape right over here, it has sides that are triangles. You see this up here is a triangle and you see this down here is a triangle, although they’re connected by these rectangular sides. This is actually what’s called a triangular prism. In a square pyramid, the faces which are triangles all meet at a point.

What 3D shape has 10 faces and 16 vertices?

There are infinitely many deltahedra, but of these only eight are convex, having 4, 6, 8, 10, 12, 14, 16 and 20 faces. The number of faces, edges, and vertices is listed below for each of the eight convex deltahedra….Non-convex forms. What 3D shape has 8 sides? 3. An octagonal prism is an eight-sided 3-D shape. What is a 16 sided 3d object called?

What does psalm Chapter 133 mean?

The themes of brotherly love and unity in verse 1 have been interpreted various ways. Rashi states that when the Jewish people are united, God joins together with them. Matthew Henry suggests that David is directing this call for unity to the sons of his many wives, or to “the benefit of the communion of saints”.

What does the Bible say about our footsteps?

This is what the LORD says: “Stand at the crossroads and look; ask for the ancient paths, ask where the good way is, and walk in it, and you will find rest for your souls. But you said, ‘We will not walk in it. ‘ A man’s heart plans his course, but the LORD determines his steps.

Where in the Bible does it say Order my steps?

Psalm 119:133
“Order my steps in thy word: and let not any iniquity have dominion over me.” Psalm 119:133 “Order My Steps” is a verse-by-verse journey through the longest chapter in the Bible.

Who wrote orphan psalms?

According to Jewish tradition, the Book of Psalms was composed by the First Man (Adam), Melchizedek, Abraham, Moses, Heman, Jeduthun, Asaph, and the three sons of Korah.

How good and pleasant it is when God’s people live together in unity Psalm 133 1 NIV?

Psalm 133 1 Of David. How good and pleasant it is when brothers live together in unity! It is like precious oil poured on the head, running down on the beard, running down on Aaron’s beard, down upon the collar of his robes. It is as if the dew of Hermon were falling on Mount Zion.

Where there is unity God commands a blessing?

In this “high priestly prayer”, the longest recorded prayer of Jesus (this is just a portion of it), Jesus gives us his vision for unity as he prays that the hearts of those who follow him will be as one as he is one with the Father.

What does the Bible say about following Jesus footsteps?

The scriptures encourage us to become like Jesus Christ and “to walk, even as he walked” (1 John 2:6). But taking that first step can be difficult—especially when you don’t know how to begin. You’ll find help in Preach My Gospel: A Guide to Missionary Service.

What Bible verse says when you saw one set of footprints?

The Lord replied, “My precious, precious child. I love you, and I would never, never leave you during your times of trial and suffering. When you saw only one set of footprints, It was then that I carried you.

What happens when God orders your steps?

First, God has an order for your steps. God is directing your steps so you can fulfill the plan He has for you. As you allow, He will show you the right choices and lead you down the best path. He will show you which step to take to keep you going the right way.

Does God really order our steps?

Psalm 37:23-24: “The Lord directs the steps of the godly. He delights in every detail of their lives. Though they stumble, they will never fall, for the Lord holds them by the hand.” Proverbs 16:9: “We can make our plans, but the Lord determines our steps.”

What is the shortest book in the Bible?

Obadiah
Obadiah is one of the Twelve Minor Prophets in the final section of Nevi’im, the second main division of the Hebrew Bible. The text consists of a single chapter, divided into 21 verses, making it the shortest book in the Hebrew Bible. The book concerns the divine judgment of Edom and the restoration of Israel.

What are the 4 types of Psalms?

Psalms of confidence. Psalms of Confidence describe the author’s trust and faith in God, typically with a central image or theme.

Psalms of Kingship. Psalms of Kingship present a king as the major focus.

Psalms of Remembrance.

Psalms of Wisdom.

How do you say Discipulus?

Pronunciation

1. (Classical) IPA: /disˈki.pu.lus/, [d̪ɪs̠ˈkɪpʊɫ̪ʊs̠]
2. (Ecclesiastical) IPA: /diʃˈʃi.pu.lus/, [d̪iʃˈʃiːpulus]
3. Audio (Classical) 0:02. (file)

How are Latin words pronounced?

Standardize your pronunciations of other consonants. Unlike in English, each Latin letter is almost always pronounced in a consistent way: C is always hard, as in “cat.” cum (with) = koom. G is always hard, as in “goal.” ago (I drive) = ah-go. S is always a voiceless “s” sound as in “snake.” spuma (foam) = spoo-ma.

How do you pronounce Vivere in Latin?

Pronunciation

1. IPA: /ˈvi.ve.re/
2. Audio. 0:02. (file)
3. Rhymes: -ivere.
4. Hyphenation: vì‧ve‧re.

What word comes from the Latin word Facere?

Several unusual English -fy verbs come from Latin compounds in -facere, –factus. Thus satisfy (L satis-facere, “to make enough”), satisfaction (L satis-fact-io); putrefy (L putre-facere, “to make rotten”), putrefaction; and liquefy (L lique-facere, “to make liquid”), liquefaction.

What conjugation is Facere?

THIRD CONJUGATION VERB

	ACTIVE	ACTIVE
	IMPERATIVE	INFINITIVE
present	face, facite	facere
perfect		fecísse
future		factúrus -a -um esse

Is Discipuli singular or plural?

Translation

	Singular	Plural
Nominative	Discipulus	Discipuli
Genitive	Discipuli	Discipulorum
Dative	Discipulo	Discipulis
Accusative	Discipulum	Discipulos

What is the meaning of Vincere?

to win
(transitive, intransitive) to win [auxiliary avere] (transitive) to vanquish, to conquer, to defeat.

What is rat tail collagen?

General description. Rat Tail Collagen Coating Solution (20 ML) enhances cell attachment and proliferation, or promotion of cell-specific morphology and function. It is commonly used for endothelial cells, hepatocytes, muscle cells, and a variety of other cell types.

How do you dilute rat tail collagen?

Dilute material to 50 µg/ml using 0.02N acetic acid. Collagen is insoluble at neutral pH. Add sufficient diluted Collagen Type I to coat dishes with 5 µg/cm2 (e.g. 1-2 ml of the above solution is sufficient to cover a 35 mm dish). Incubate at room temperature for one hour.

What does collagen do in cell culture?

Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.

How do you store collagen coated plates?

Label and date tube, store at 4°C, use within 7 days. Incubate overnight at room temperature to ensure ensure that collagen has adhered to the plastic. If flasks/plates are urgently needed incubate 2-3 hours at 37°C. After the incubation, excess collagen may be taken off and used to coat other surfaces.

How do you dissolve collagen type 1?

Collagen Type I coating protocol for culture ware Add collagen to acetic acid (refer to Table 1) to obtain 0.1% (w/v) collagen solution. Stir at room temperature for 1-3 hours until dissolved. Collagen solution should be diluted 10-fold to obtain a working concentration of 0.01%.

Can you sterile filter collagen?

Collagen solutions may be sterilized by dialysis in a 0.25% acetic acid and 0.5% chloroform solution. We do not recommend sterilizing the collagen solution by membrane filtration.

How long does it take to coat collagen?

Results

	Pipette controller	DOSE IT
Dispense collagen	13 min	6 min
Aspirate collagen	15 min	12 min
Washing (2x)	60 min	35 min
Total	88 min	53 min

How do you sterilize collagen powder?

Collagen solutions may be sterilized by dialysis in a 0.25% acetic acid and 0.5% chloroform solution. We do not recommend sterilizing the collagen solution by membrane filtration. We have found substantial protein loss by this method. Coating of tissue culture plastic dishes may be performed by air-drying.

What is the concentration of rat tail collagen?

The rat tail collagen I offered here is supplied at a concentration of 3 mg/mL, providing the flexibility to dilute to lower concentrations. For Research Use Only. Not for use in diagnostic procedures.

What is the concentration of GIBCO collagen I and rat tail?

We offer Collagen I Rat Tail and Collagen I Bovine at 5 mg/mL concentration which gives the flexibility to dilute to lower concentrations. Figure shows Gibco Collagen I and collagen from two other suppliers were prepared at the concentrations stated and allowed to solidify in individual wells of a 24-well plate.

What is rat tail tendon collagen used for?

Rat tail tendon collagen is used in tissue engineering especially in the generation of 3-D scaffolds based gels. It has low antigenicity and is compatible with human gingival fibroblasts and human oral keratinocytes. Sterile solution prepared from rat tail tendons by a modification of the published extraction method 1.

What is the shelf life of collagen solutions?

Collagen solutions should be stored in the refrigerator at 2-8 °C for up to 1 year, unless it becomes contaminated. Why am I seeing some insolubles in my collagen solution? The solubility specifications for this product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid (or 0.1 N acetic acid).