Is 18S rRNA as housekeeping gene?
Cytokine analysis revealed that only normalization to 18S rRNA gave a result that satisfactorily reflected their mRNA expression levels per cell. In conclusion, 18S rRNA was the most stable housekeeping gene and hence superior for normalization in comparative analyses of mRNA expression levels in human T lymphocytes.
Are rRNA genes housekeeping genes?
In summary we concluded that18S rRNA is a suitable housekeeping gene, while ACTB and GAPDH are not as reliable for normalising qRT-PCR data from influenza virus infected HBECs, PTECs, chicken and duck cells.
What is the purpose of the housekeeping gene in RT PCR?
Housekeeping genes are cellular maintenance genes which regulate basic and ubiquitous cellular functions. In many RT-qPCR reactions, these genes are used as internal control genes without proper validation.
Why are housekeeping genes used?
Housekeeping genes are typically constitutive genes that are required for the maintenance of basal cellular functions that are essential for the existence of a cell, regardless of its specific role in the tissue or organism.
What does the housekeeping gene do?
Housekeeping genes are involved in basic cell mainte- nance and, therefore, are expected to maintain constant expression levels in all cells and conditions. Identification of these genes facilitates exposure of the underlying cellular infrastructure and increases understanding of various structural genomic features.
How does a housekeeping gene work?
Is 18S rRNA the most stable housekeeping gene for normalization?
Cytokine analysis revealed that only normalization to 18S rRNA gave a result that satisfactorily reflected their mRNA expression levels per cell. In conclusion, 18S rRNA was the most stable housekeeping gene and hence superior for normalization in comparative analyses of mRNA expression levels in human T lymphocytes.
Is 18S rRNA a good housekeeping gene for influenza virus infection?
In summary we concluded that18S rRNA is a suitable housekeeping gene, while ACTB and GAPDH are not as reliable for normalising qRT-PCR data from influenza virus infected HBECs, PTECs, chicken and duck cells.
Should we use 18S rRNA for normalising mRNA expression?
Another criticism of using 18S rRNAfor normalising mRNA expression is that the ribosomal fraction may not truly represent the overall cellular mRNA population [29]. However, influenza virus infection inhibits cellular macromolecular synthesis (shut-off) and causes global gene expression changes [30-35] affecting mRNA levels of many genes.
What is the most suitable gene for housekeeping?
Coefficient of correlation [r] HBECs 0.962 0.871 0.709 0.543 PTECs 0.843 0.743 0.741 0.73 Chicken lung cells 0.944 0.832 0.845 Duck lung cells 0.836 0.792 0.542 Open in a separate window Pairwise correlation and regression analysis using Bestkeepercorrelation analysis (n=12) found 18S rRNAas the most suitable housekeeping gene.