What is rat tail collagen?
General description. Rat Tail Collagen Coating Solution (20 ML) enhances cell attachment and proliferation, or promotion of cell-specific morphology and function. It is commonly used for endothelial cells, hepatocytes, muscle cells, and a variety of other cell types.
How do you dilute rat tail collagen?
Dilute material to 50 µg/ml using 0.02N acetic acid. Collagen is insoluble at neutral pH. Add sufficient diluted Collagen Type I to coat dishes with 5 µg/cm2 (e.g. 1-2 ml of the above solution is sufficient to cover a 35 mm dish). Incubate at room temperature for one hour.
What does collagen do in cell culture?
Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.
How do you store collagen coated plates?
Label and date tube, store at 4°C, use within 7 days. Incubate overnight at room temperature to ensure ensure that collagen has adhered to the plastic. If flasks/plates are urgently needed incubate 2-3 hours at 37°C. After the incubation, excess collagen may be taken off and used to coat other surfaces.
How do you dissolve collagen type 1?
Collagen Type I coating protocol for culture ware Add collagen to acetic acid (refer to Table 1) to obtain 0.1% (w/v) collagen solution. Stir at room temperature for 1-3 hours until dissolved. Collagen solution should be diluted 10-fold to obtain a working concentration of 0.01%.
Can you sterile filter collagen?
Collagen solutions may be sterilized by dialysis in a 0.25% acetic acid and 0.5% chloroform solution. We do not recommend sterilizing the collagen solution by membrane filtration.
How long does it take to coat collagen?
Results
| Pipette controller | DOSE IT | |
|---|---|---|
| Dispense collagen | 13 min | 6 min |
| Aspirate collagen | 15 min | 12 min |
| Washing (2x) | 60 min | 35 min |
| Total | 88 min | 53 min |
How do you sterilize collagen powder?
Collagen solutions may be sterilized by dialysis in a 0.25% acetic acid and 0.5% chloroform solution. We do not recommend sterilizing the collagen solution by membrane filtration. We have found substantial protein loss by this method. Coating of tissue culture plastic dishes may be performed by air-drying.
What is the concentration of rat tail collagen?
The rat tail collagen I offered here is supplied at a concentration of 3 mg/mL, providing the flexibility to dilute to lower concentrations. For Research Use Only. Not for use in diagnostic procedures.
What is the concentration of GIBCO collagen I and rat tail?
We offer Collagen I Rat Tail and Collagen I Bovine at 5 mg/mL concentration which gives the flexibility to dilute to lower concentrations. Figure shows Gibco Collagen I and collagen from two other suppliers were prepared at the concentrations stated and allowed to solidify in individual wells of a 24-well plate.
What is rat tail tendon collagen used for?
Rat tail tendon collagen is used in tissue engineering especially in the generation of 3-D scaffolds based gels. It has low antigenicity and is compatible with human gingival fibroblasts and human oral keratinocytes. Sterile solution prepared from rat tail tendons by a modification of the published extraction method 1.
What is the shelf life of collagen solutions?
Collagen solutions should be stored in the refrigerator at 2-8 °C for up to 1 year, unless it becomes contaminated. Why am I seeing some insolubles in my collagen solution? The solubility specifications for this product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid (or 0.1 N acetic acid).