What are the different types of plots in flow cytometry?
Flow cytometry data is typically represented in one of two ways: histograms, which measure or compare only a single parameter, and dot-plots which compare 2 or 3 parameters simultaneously on a two- or three-dimensional scatter-plot.
What is a contour plot in flow cytometry?
Contour plots display the relative frequency of the populations, regardless of the number of events collected. The plots show an enriched dendritic cell (DC) population from mouse spleen on which only a few hundred events could be collected. To the left is a regular dot plot showing all events.
How do you read a flow cytometry histogram?
The X-axis is the amount of red fluorescence. The more red fluorescence a cell emits, the farther to the right the cell data will appear on the histogram. The Y-axis is the amount of blue fluorescence. The more blue fluorescence a cell emits, the cell data will appear closer to the top on the histogram.
What is FITC in flow cytometry?
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. First described in 1942, FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure.
What are contour plots used for?
Contour plots (sometimes called Level Plots) are a way to show a three-dimensional surface on a two-dimensional plane. It graphs two predictor variables X Y on the y-axis and a response variable Z as contours. These contours are sometimes called z-slices or iso-response values.
What is flow cytometry?
Flow cytometry is a technology that provides rapid multi-parametric analysis of single cells in solution. Flow cytometers utilize lasers as light sources to produce both scattered and fluorescent light signals that are read by detectors such as photodiodes or photomultiplier tubes.
What is a density plot used for in microbiology?
To further analyze your cell population, density plots-or bivariate scatter plots-allow for combinations of dyes to be analyzed simultaneously. This is particularly effective in heterogeneous fluid, like blood or saliva, where the number of different cell populations within the sample is high.
How is the intensity of a distribution represented in flow cytometry?
In flow cytometry, the intensity of a distribution can be represented by the position of the “centre” of the distribution. The “centre” is usually represented mathematically by the mean, median or peak channel number.
How is data acquired from a flow cytometer?
draw regions and set gates (see below) to be used during data acquisition. If the flow cytometer can sort cells, the computer controls the sorting process. As data are acquired, they written to the hard drive to create a file of data, often referred to as ‘listed data’.