What cells express LysM?
While LysP is expressed in the Paneth cells of the small intestine, LysM is exclusively expressed in myelomonoytic cells including monocytes, macrophages and granulocytes in mice (10). LysM is therefore served as a marker for myelomonoytic cells.
What is CD4 Cre?
CD4-Cre transgenic mice contain CD4 enhancer, promoter and silencer sequences driving the expression of a Cre recombinase gene. Hemizygotes are viable and fertile. Specifically, Cre recombinase expression is observed in CD4-expressing T cells during sequential stages of T cell development in lymphoid tissues.
Do all dendritic cells express CD11c?
CD11c is commonly used as mouse DC marker. However, CD11c is not restricted to DCs, but also expressed by other immune cells, such as macrophages in the alveoli (Gonzalez-Juarrero et al., 2003) and adipose tissues (Brake et al., 2006). In humans, both monocytes and dendritic cells express CD11c (Collin et al., 2012).
Are LysM-Cre mice deleted in dendritic cells?
It is known that LysM-cre mice are deleted in monocytes, macrophages and neutrophils, but what about dendritic cells? Join ResearchGate to ask questions, get input, and advance your work. yes and no. Some people report no change in the DC population and some yes.
What are lysmcre mice used for?
These LysMcre mice may be useful for Cre-lox studies of the myeloid cell lineage (monocytes, mature macrophages and granulocytes) and the innate immune response. 004781 – LysMcre Strain Details If you need help with your order, contactCustomer Support
What is the difference between LysM-CRE and GE-CRE?
LysM-cre promotes significant deletion in macrophages, but also in neutrophils. Like LysM-cre, GE-cre promotes significant deletion in neutrophils and macrophages. F4/80-cre:ROSA-EYFP mice show ~50% deletion in peritoneal macrophages, but do not exhibit deletion in other types of macrophages.
What is the lysmcre knock-in allele?
The LysMcre knock-in allele has a nuclear-localized Cre recombinase inserted into the first coding ATG of the lysozyme 2 gene (Lyz2); both abolishing endogenous Lyz2 gene function and placing NLS-Cre expression under the control of the endogenous Lyz2 promoter/enhancer elements.