What does filipin stain?
Filipin staining is a generally accepted tool for detection of cholesterol deposits in NPC cells [3, 39–41]. We aimed to characterize PFO as another convenient probe for visualization and analysis of cholesterol in cells.
What does filipin bind?
Filipin is a naturally fluorescent polyene antibiotic that binds to cholesterol but not to esterified sterols. Thus, it is useful for detecting free (i.e., unesterified) cholesterol in biological membranes.
How does filipin bind to cholesterol?
Filipin is known to bind to various sterol derivatives and phospholipids on thin-layer chromatograms. Biochemical evidence is presented showing that filipin also binds to GM1 with an affinity similar to that for cholesterol, with a corresponding fluorescent reaction.
Is cholesterol a fluorescent?
NBD- and Dansyl-cholesterol NBD-cholesterol with the fluorophore at carbon 25 has been shown to be mistargeted in cells to mitochondria (Mukherjee et al., 1998). Dansyl-cholesterol is another fluorescent cholesterol analog used in cellular studies (Wiegand et al., 2003, Huang et al., 2010).
What is NBD cholesterol?
This fluorescent analog, NBD cholesterol, is an environment-sensitive probe that localizes in the membrane’s interior and is useful for investigating lipid transport processes as well lipid-protein interactions.
What is TopFluor?
TopFluor is a fluorescent molecule characterized by its dipyrromethene boron difluoride moiety, which has been covalently attached to different lipid molecules, including the C11 acyl chain tail of C1P as shown in figure 1A.
What does bodipy FL stain?
BODIPY FL dye is a green fluorescent dye with unique hydrophobic properties ideal for staining lipids, membranes, and other lipophilic compounds.
How do you store bodipy?
Recipes
- Prepare 5 mM BODIPY stock solution. Dissolve 1.3 mg BODIPY in 1 ml DMSO and can be stored at −20 °C.
- 2 μM BODIPY staining solution can be prepared by diluting stock solution 1:2,500 in PBS.
What does BODIPY stain for?
Additionally, since BODIPY 505/515 staining is non-destructive, it allows samples to be used for other analysis after lipid determination. This high quantum yield dye has been used for staining lipid-containing yolk platelets in live zebrafish embryos (Cooper et al., 2010).
What are the emission spectra of hydrogen?
Important atomic emission spectra. The spectral series of hydrogen, on a logarithmic scale. The emission spectrum of atomic hydrogen has been divided into a number of spectral series, with wavelengths given by the Rydberg formula. These observed spectral lines are due to the electron making transitions between two energy levels in an atom.
What is the Balmer series of hydrogen emission spectrum?
The four visible hydrogen emission spectrum lines in the Balmer series. H-alpha is the red line at the right. The Balmer series includes the lines due to transitions from an outer orbit n > 2 to the orbit n’ = 2. Named after Johann Balmer, who discovered the Balmer formula, an empirical equation to predict the Balmer series, in 1885.
What causes the lines in the emission spectrum of hydrogen?
The emission spectrum of atomic hydrogen is divided into a number of spectral series, with wavelengths given by the Rydberg formula. These observed spectral lines are due to the electron making transitions between two energy levels in an atom.
What is the Rydberg formula for hydrogen emission spectrum?
The emission spectrum of atomic hydrogen is divided into a number of spectral series, with wavelengths given by the Rydberg formula: 1 λvac =RZ2( 1 n12 − 1 n22) 1 λ v a c = R Z 2 ( 1 n 1 2 − 1 n 2 2),