What is a non hydrolyzable ATP analog?
A relatively stable, slowly hydrolyzable ATP analog that can substitute for ATP in various kinase reactions. Used as a substrate and as an inhibitor of ATP-dependent enzyme systems. View Pricing.
What are ATP analogues?
In energy-transducing reactions, ATP is normally hydrolysed between the ß and γ phosphate groups, and modification of this region produces slowly hydrolysable or non-hydrolysable analogues (e.g. AP. PNP). These derivatives can be used to assess the role of binding energy in the transduction process.
What is Atpγs?
Adenosine 5′-O-(3-thio)triphosphate (ATPγS) is a substrate for the nucleotide hydrolysis and RNA unwinding activities of eukaryotic translation initiation factor eIF4A – PMC.
What does hydrolyzable mean?
capable of undergoing hydrolysis
hydrolyzable – capable of undergoing hydrolysis.
What does AMP PNP do?
AMP PNP is a non-hydrolyzable AMP analog. Kir6 (KATP) channel blocker. Inhibits fast axonal transport and stabilizes the interaction of membranous organelles with microtubules.
Which has more energy ATP or ADP?
Thus, ATP is the higher energy form (the recharged battery) while ADP is the lower energy form (the used battery). When the terminal (third) phosphate is cut loose, ATP becomes ADP (Adenosine diphosphate; di= two), and the stored energy is released for some biological process to utilize.
Which is non Hydrolysable?
The non-hydrolyzable plastics PE, PP, PS, and PVC (in italics) are more resistant to enzymatic degradation because they require oxidation of inert backbone chains. Their monomer structures in high molecular weight polymer chains are shown adjacent to bars.
What does the word Hydrolyse mean?
: a chemical process of decomposition involving the splitting of a bond and the addition of the hydrogen cation and the hydroxide anion of water.
What is the structure of AMP-PNP?
Phosphoaminophosphonic acid-adenylate ester
| PubChem CID | 33113 |
|---|---|
| Structure | Find Similar Structures |
| Molecular Formula | C10H17N6O12P3 |
| Synonyms | AMP-PNP ADENYLYL IMIDODIPHOSPHATE gamma-Imino-ATP AMPPNP 25612-73-1 More… |
| Molecular Weight | 506.20 |
What are the non hydrolysable analogs of ATP?
Non-hydrolysable analogues are also useful in crystallographic studies, as are the stable complexes formed between protein-bound ADP and phosphate analogues, such as vanadate. Another route to making a stable ATP state is the use of Co (III) or Cr (III) metal substitutes that display very slow ligand-exchange rates.
What is the non-hydrolyzable ATP test kit?
The Non-hydrolyzable ATP Test Kit contains a set of 5 typical non-hydrolyzable Adenosine-5′-triphosphate analogs. The position of the modification within the triphosphate exerts influence on its binding affinity to a particular enzyme and its hydrolysis rate.
Why do we use non-hydrolyzable nucleotide analogs?
Since natural nucleotides are hydrolyzed at rates usually preventing such studies, nucleotide analogs are used that are hydrolyzed at much smaller rates or that are even completely resistant to hydrolysis. The Non-hydrolyzable ATP Test Kit contains a set of 5 typical non-hydrolyzable Adenosine-5′-triphosphate analogs.
How easy is it to make ATP analogs?
The ease of synthesis of ATP analogues varies widely: some require specialist chemical skills and facilities, whereas others can be made by straightforward labelling reactions under aqueous conditions.