What is anti HA antibody?
Anti-HA antibody is specific for N- or C-terminal HA-tagged fusion proteins. The product may detect cross-reacting bands in certain mammalian cells. The immunizing HA peptide (Product No.I2149) specifically inhibits the staining of the HA-tagged protein band.
What is the molecular weight of HA tag?
1.1 kDa
The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa and is a linear epitope derived from amino acids 98-106 of the HA protein.
How do you purify HA tagged protein?
How do you purify HA-tagged proteins? HA-tagged recombinant protein can be affinity purified directly from a cell culture lysate or supernatant. The HA-tagged protein binds to the HA-tag specific monoclonal antibody conjugated on an agarose gel.
What is a V5 tag?
V5 tag is a short peptide tag for detection and purification of proteins. The V5 tag can be fused/cloned to a recombinant protein and detected in ELISA, flow cytometry, immunoprecipitation, immunofluorescence, and Western blotting with antibodies and Nanobodies.
What is a Myc DDK tag?
A Myc-tag is a polypeptide protein tag derived from the c-myc gene product that can be fused to either the C- or N-terminus of a protein through recombinant DNA technology. It can facilitate the affinity purification of the tagged protein, as well as the isolation and detection of the tagged protein.
What is the HA tag sequence?
The HA tag’s nucleotide sequence is: 5′ TAC CCA TAC GAT GTT CCA GAT TAC GCT 3′ or 5′ TAT CCA TAT GAT GTT CCA GAT TAT GCT 3′ The HA tag’s amino acid sequence is: YPYDVPDYA.
What is ha in bio?
From Wikipedia, the free encyclopedia. Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human influenza virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors.
How big is a flag-tag?
2. Alternatives to the Flag-tag
| Feature | Flag® Tag |
|---|---|
| Size of the tag in Da | 1012.0 Da |
| Affinity matrix | Anti Flag® antibody (approx. 150 kDa) |
| Elution conditions | Flag® peptide, low pH, or tag cleavage |
| Specificity of interaction (KD) | 100 nM (3) |
What is HA sequence?