What is capillary Electrochromatography used for?
Capillary electrophoresis aims to separate analytes on the basis of their mass-to-charge ratio by passing a high voltage across ends of a capillary tube, which is filled with the analyte.
What is capillary HPLC?
Capillary HPLC is a specialized form of LC employed with reduced diameter columns to improve sensitivity by reducing on-column sample dilution. • Cap LC does not require large sample amounts and may be qualitative and quantitative, often employing mass spec as a detector.
What is capillary electrophoresis used for in forensic science?
Capillary electrophoresis (CE) is used in the ATF Forensic Science Laboratories for the analysis of inorganic ions commonly encountered in post-blast residues including monomethylamine (MMA) and benzoate ions. Monomethylamine nitrate is found in Tovex, a water gel explosive.
What are the advantages of capillary electrophoresis?
Capillary electrophoresis (CE) provides a number of advantages for analysts, including high separation efficiency, short analysis times, low waste generation, and a diverse range of applications.
What is the mobile phase used in capillary electrophoresis?
The heart of capillary electrophoresis (CE) is electroosmotic flow (EOF). This is the mobile phase “pump” in CE. Unlike gas chromatography (GC), there is no pressurized gas acting as the mobile phase in CE.
Is capillary column used in HPLC?
LC/MS is best done with capillary HPLC. Capillary HPLC uses smaller column internal diameters than conventional HPLC. Smaller ID columns, for fixed amounts of injected material, produce taller peaks. Taller peaks provide better detection limits for mass spectrometry and other concentration sensitive detectors.
What are HPLC columns made of?
Common packing materials in HPLC columns include silica or hydroxyapatite media and polymeric resins such as polystyrene divinylbenzene. The use of smaller-diameter beads generally results in improved separation sensitivity due to the increased surface area.
What controls are used in capillary electrophoresis?
Capillary Electrophoresis and Electrochromatography Electrophoretic mobility is controlled by an external electric field and selectivity can be manipulated by a number of factors including solvent pH, ionic strength, and other additives.
What is Capillary electrochromatography?
Capillary electrochromatography is a combination of two analytical techniques, high-performance liquid chromatography and capillary electrophoresis. Capillary electrophoresis aims to separate analytes on the basis of their mass-to-charge ratio by passing a high voltage across ends of a capillary tube, which is filled with the analyte.
Does temperature affect electroosmotic flow in capillary electrochromatography?
Wen et al.[16] extensively studied dynamics in capillary electrochromatography and found linear Van ‘t Hoff plot for the Spherisorb ODS column; electroosmotic flow (EOF) increased with temperature as a result of the decrease of viscosity of the mobile phase.
What is the retention mechanism in capillary electrochromatography?
The proper understanding of retention mechanism in capillary electrochromatography is a fundamental problem for this recently developed separation technique. At first, it is believed that partitioning of neutral solutes on the reversed-phase CEC is identical or at least very similar to reversed-phase HPLC.
Can Capillary electrochromatography separate three basic drugs on reversed phase?
9.4 Conclusions A capillary electrochromatography method was developed for the separation of three basic drugs on reversed-phase stationary phases after a simple derivatization procedure of one of the compounds.
