What is cross-linking in immunology?
Crosslinking is the process of chemically joining two or more molecules by a covalent bond. Crosslinking reagents (or crosslinkers) are molecules that contain two or more reactive ends capable of chemically attaching to specific functional groups (primary amines, sulfhydryls, etc.)
What is cross-linking of antibodies?
The antibody-bead cross-linking process generates a reusable resource of antibody and beads, commonly referred to as an antibody-specific resin, and can be repeatedly used for the immunoprecipitation of specific proteins if treated and stored correctly.
What is cross-linking methods?
Cross-linking is a method to covalently bond two or more molecules in a sample by using a chemical or a physical agent.
What are cross-linked polymers explain with example?
Crosslinked polymers can be found in both synthetic polymers and as naturally occurring polymers. Crosslinks can be formed from chemical reactions using crosslinking reagents. The most common example of crosslinked polymers is the vulcanized rubber.
What causes cross linking?
Cross-links can be formed by chemical reactions that are initiated by heat, pressure, change in pH, or irradiation. For example, mixing of an unpolymerized or partially polymerized resin with specific chemicals called crosslinking reagents results in a chemical reaction that forms cross-links.
What is cross linking in biomaterials?
Crosslinking has been the most common approach to overcome the limitations of biomaterials [9,10]. Crosslinkers interconnect molecules, increase mo- lecular weight, and generally provide higher mechanical properties and improved stability.
What is the major purpose of cross-linking in polymers?
Introduction. Chemical cross-linking has been widely used to alter the physical properties of polymeric materials, the vulcanization of rubber being a prototypic example. Linking of polymer chains through chemical linkages gives a material a more rigid structure and potentially a better-defined shape.
What is cross-linking in biology?
Crosslinking is the process of chemically joining two or more molecules by a covalent bond. Crosslinking reagents (or crosslinkers) are molecules that contain two or more reactive ends capable of chemically attaching to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules.
What is meant by crosslinking proteins?
Crosslinking proteins Crosslinking is the process of chemically joining two or more molecules by a covalent bond. Crosslinking reagents (or crosslinkers) are molecules that contain two or more reactive ends capable of chemically attaching to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules.
What is crosslinking reagent?
Crosslinking is the process of chemically joining two or more molecules by a covalent bond. Crosslinking reagents (or crosslinkers) are molecules that contain two or more reactive ends capable of chemically attaching to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules.
What is the role of crosslinking in atherosclerosis?
In biological context crosslinking could play a role in Atherosclerosis through advanced glycation end-products which have been implicated to induce crosslinking of collagen which may lead to vascular stiffening. The interactions or mere proximity of proteins can be studied by the clever use of crosslinking agents.
What are the two ways of crosslinking?
The first is through free radical or oxidizing reactions assisted by an increased temperature and the presence of air. UV light or other radiation sources may also promote crosslinking. Another mechanism is a chemical reaction such as through the condensation of an alcohol or an amine with a carboxylic acid.