What is HyClone FBS?

Table of Contents

What is HyClone FBS?

HyClone characterized fetal bovine serum (FBS) is produced using true pool technology, which minimizes bottle-to-bottle variability. This FBS product is filtered through three sequential 100 nm (0.1 µm) pore size-rated filters. Each lot of FBS is subjected to virus panel testing according to 9 CFR 113.53.

Why do we heat inactivate FBS?

A common treatment of FBS is heat-inactivation, where FBS is heated at 56°C for 30 minutes in a water bath with occasional shaking. The purpose is to inactivate whatever components of the complement system are present in the FBS [24], and other potential unknown inhibitors of cell growth.

Is Gibco FBS Heat inactivated?

Gibco heat-inactivated FBS is now available in our popular Gibco One Shot 50 mL bottle, which enables you to stop aliquoting and avoid bath time in, yes, just one shot.

What is the concentration of FBS?

Typically used at a concentration of 5–10% in a basal medium. FBS composition is undefined, and varies from lot-to-lot.

What is in bovine calf serum?

Bovine Calf Serum (BCS) is defined as the liquid fraction of clotted blood derived from healthy, slaughtered bovine calves, aged from 20 days up to 12 months, deemed fit for human consumption by ante-and/or post- mortem inspection.

What happens if you heat inactivate serum for too long?

Notes: Heating the serum for longer than 30 minutes or higher than 56°C will have an adverse effect on the efficacy of the serum and will most likely cause an increase in the amount of cryo-precipitate that might form.

How long does FBS last in fridge?

Liquid FBS and other serum may be stored refrigerated (2 to 8 °C) up to four weeks.

How long is FBS good for?

What is FBS solution?

Fetal bovine serum is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells. This is due to it having a very low level of antibodies and containing more growth factors, allowing for versatility in many different cell culture applications.

How is FBS made?

Abstract. Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia.

What proteins are in FBS?

The major component of FBS is serum albumin which constitutes 60 to 67% of total proteins. Other abundant proteins include cone cGMP-specific 3′,5′-cyclic phosphodiesterase R-subunit, alpha-1-antiproteinase, plasminogen and lactoperoxidase.

Can you thaw FBS at room temperature?

Thaw FBS in the fridge. Allow serum to acclimate at room temperature for a minimum of 10 minutes or refrigerate overnight at 2° C to 8° C. The serum may then be completely thawed at room temperature. Or, you may choose to proceed by thawing the serum in a water bath at 37° C.

How is HyClone defined FBS filtered?

HyClone Defined FBS is filtered through serial 40 nm (0.04 μm) pore size-rated filters, which are the most retentive filters used in commercial FBS production. This type of filtration is a practical, cost-competitive method of viral load reduction.

What is HyClone serum used for?

Cytiva HyClone™ Characterized Fetal Bovine Serum (U.S.) Used as serum-supplement for the in vitro cell culture of eukaryotic cells Manufacturer: Cytiva SH30071.03 Need it Now? See alternate available products Sera should be stored at -10°C or lower. Once thawed, sera should be stored at 2 to 8°C. One moment while we fetch your results.

What is HyClone fetal bovine serum?

Is HyClone serum traceability-certified?

HyClone serum processes are traceability-certified by the International Serum Industry Association (ISIA). In addition, Cytiva has chosen to be an early adopter of scientific traceability by Oritain to prove the country of origin of their premium bovine serum products from the United States, Australia, and New Zealand.

https://www.youtube.com/watch?v=QlLkz1sx8w8