What is TCO chemistry?

What is TCO chemistry?

TCO click chemistry is the 3rd generation click chemistry that is widely used in labeling and bioconjugation due to its fast speed and biocompatibility.

Does TCO react with azide?

In conclusion, we describe the testing and in-cell validation of a tetrazine–TCO reaction pair that is orthogonal to azide–cyclooctyne cycloaddition chemistry. The chosen pairs were able to react concurrently in the same culture to fluorescently label two different cancer cell types.

How is Yellow 5 made?

Tartrazine, also referred to as FD&C yellow #5, is an artificial (synthetic) food dye. It is one of several azo food dyes that are made from petroleum products. Artificial food dyes are used to make foods more aesthetically appealing from a visual standpoint.

Is FD&C Yellow 6 Safe?

FD&C Yellow 6 is a synthetic dye produced from petroleum; this dye is FDA-approved for use in food, pharmaceuticals and cosmetics.

What are the applications of tetrazine cycloaddition reactions?

The use of tetrazine cycloaddition reactions is not limited only to bio-orthogonal chemistry. It also finds application in materials chemistry. It was used to allow an easy modification of a highly ordered pyrolytic 2D graphite surface under ambient conditions ( 14CM5058 ).

What is tetrazine ligation?

The tetrazine ligation is an inverse electron demand Diels-Alder (IEDDA) reaction between s-tetrazines and alkenes/alkynes. It has gathered significant attention since its initial use for bioconjugation (Blackman, Royzen, & Fox, 2008).

How are tetrazines used in macromolecules?

Tetrazines were also used in the design of macromolecules. Indeed, functionalized O 6 -corona [3]arene [3]tetrazines were obtained by a condensation reaction between N -functionalized 3,6-dihydroxyphthalimides and 3,6-dichlorotetrazine in a one-pot manner.

How many isomers of tetrazine are there?

While all three different isomers of tetrazines are conceivable (v-tetrazines, as-tetrazines, and s-tetrazines), symmetrical 1,2,4,5-tetrazine is the one most commonly used in modern fluorescence research (Fig. 65 ). Fig. 65. Structures of the tetrazine isomers (v-tetrazine, as-tetrazine, and s-tetrazine).