What is the pKa of HEPES?
N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES) is a zwitterionic buffer with a pKa of 7.3 at 37 °C.
How do you change the pH of a HEPES buffer?
Adjust the pH to 7.4 with HCl or NaOH. HEPES buffer can be stored refrigerated for several weeks.
What is HEPES buffer?
HEPES buffer or (4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid) is a biological buffer used in cell culture. In chemistry, a buffer is a solution that contains an acid and a base or salt.
Is HEPES a phosphate buffer?
And the traditional buffers used in the past , Such as carbonate buffer and phosphate buffer, are usually not suitable for many biological experiments. These reagents cannot effectively buffer above pH 7.5….HEPES VS PBS (phosphate buffered saline)
| HEPES | PBS | |
|---|---|---|
| Valid pH range | 6.8-8.2 | 5.8-8.0 |
| Biological toxicity | Not biologically toxic | Not biologically toxic |
What’s in HEPES buffer?
HEPES buffer – or (4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid) – is a biological buffer used in cell culture. In chemistry, a buffer is a solution that contains an acid and a base or salt.
What does HEPES buffer contain?
Gibco HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent and is categorized as a “Good” buffer which derives from a set of buffers described by Dr. Norman Good and his colleagues in 1966 (Good et. al., Biochemistry 1966).
How do I make a 20mm HEPES buffer?
One typical way is prepare a stock solution of 0.5 or 1 M HEPES from solid, with the pH adjusted to 7.2 using NaOH. This is then diluted to make the 20 mM buffer.
Can HEPES buffer be autoclaved?
Some buffer materials (e.g., PIPES) do not go into solution easily and require a slightly alkaline or acid environment or heating before they will dissolve. Some buffers (e.g., MOPS and HEPES) cannot be autoclaved because they degrade upon heating.