What is the role of serine in factor Xa?
Factor Xa is a serine protease which cleaves prothrombin to generate thrombin and lies at the crossroads of the extrinsic and intrinsic coagulation pathway. Only a small amount of factor Xa is needed to generate many molecules of thrombin.
What does serine protease cleave?
Serine proteases are enzymes that cleave peptide bonds in proteins, in which serine serves as the nucleophilic amino acid at the active site.
What is the complex formed with factor Xa?
Factor Xa is the prime component of the prothrombinase complex which converts large amounts of prothrombin—the “thrombin burst”. Each molecule of Factor Xa can generate 1000 molecules of thrombin. This large burst of thrombin is responsible for fibrin polymerization to form a thrombus.
What does clotting factor Xa do?
Factor Xa inhibitors are a type of anticoagulant (blood thinning drugs) that work by binding selectively and reversibly to the clotting factor Xa. Factor Xa plays a crucial role in the blood clotting mechanism when you get an injury by forming a mesh to prevent loss of blood.
Is factor Xa a protease?
Factor Xa Protease preferentially cleaves after the arginine residue in the amino acid sequence Ile-Glu-Gly-Arg. Recommended Reaction Buffer: 20mM Tris-HCl (pH 7.4), 0.1M NaCl. Cleavage of proteins and fusion proteins containing Factor Xa Protease sites, including fusion proteins generated with the PinPoint™ Vectors.
Which factor S determines the specificity of serine proteases?
The substrate residue N-‐terminal to the cleavage site (P1) largely determines the specificity of serine proteases.
How is Factor X activated by serine protease?
Activation of factor X to a serine protease occurs predominantly by hydrolysis of the Arg194↓Ile195 bond in the heavy chain, which releases a 52-residue activation peptide to form factor Xaα.
What is factor Xa protease?
Factor Xa Protease is purified from bovine plasma and activated by treatment with the activating enzyme from Russell’s viper venom.
What is the difference between Factor X and Factor Xa?
Factor X is the zymogen of a serine protease. The activated enzyme (factor Xa) is an Arg-specific serine protease related to trypsin, but has much narrower substrate specificity.
Is Factor X a serine endopeptidase?
It is a serine endopeptidase (protease group S1, PA clan ). Factor X is synthesized in the liver and requires vitamin K for its synthesis.