What is the purpose of a Myc tag?
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism.
What is Myc tag antibody?
Myc-Tag Antibody detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. The antibody may cross-react with c-myc protein.
Is Myc an antibody?
The c-Myc Antibody confirms silencing of c-Myc expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity. Western blot analysis of extracts from HeLa, BaF3 and NBT-11 cells, using c-Myc Antibody.
How many Myc tags are there?
The Myc-tag can be used for many capture and detection applications such as immunoprecipitation, immunofluorescence and protein purification. 1. What is Myc- tag?…11. Myc-tag vs. HA-tag.
| Myc-tag | HA-tag | |
|---|---|---|
| Amino acid sequence | EQKLISEEDL | YPYDVPDYA |
| Number of amino acids | 10 | 9 |
| Molecular weight (in Da) | 1203.31 | 1102.17 |
How big is a Myc tag?
5 x 1 mg
Description
| Target: | c-Myc |
|---|---|
| Product Type: | Peptide |
| Size: | 5 x 1 mg |
| Research Area / Disease: | Epitope Tag |
| Gene ID Human: | 4609 |
How does Myc gene work?
The c-Myc gene serves as a “master regulator” of cellular metabolism and proliferation. Since it is activated by a large number of oncogenic pathways and, in turn, stimulates many of the metabolic changes that result in malignant transformation, it is truly “both the chicken and the egg”.
How does a His-tag work?
His-tag purification uses the purification technique of immobilized metal affinity chromatography, or IMAC. In this technique, transition metal ions are immobilized on a resin matrix using a chelating agent such as iminodiacetic acid.
