What is the molecular weight of dextran?
Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million.
What size molecules can pass the blood brain barrier?
Transport at the BBB. Generally, only lipid soluble (lipophilic) molecules with a low molecular weight (under 400–600 Da) and of positive charge can cross the BBB.
What is the molecular weight of dextran blue?
∼2,000 kDa
The Vo of a given column is based on the volume of effluent required for the elution of a large molecule such as blue dextran (molecular mass of ∼2,000 kDa, Catalog Number D4772).
What is the most suitable size of dextran for clinical uses?
Two dextran preparations are suitable for clinical use: dextran 40, with an average molecular weight of 40,000 and dextran 70, with an average molecular weight of 70,000. Dextran 70 is preferred for volume expansion and dextran 40 for flow-improving effects.
Is dextran inert?
Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.
Is dextran a storage polysaccharide?
The name dextran was created by Scheibler in 1874, who demonstrated dextran was a carbohydrate with the formula (C6H10O6)n and a positive optical rotation. Dextrans are polysaccharides with molecular weights ≥1,000 Dalton, which have a linear backbone of α-linked d-glucopyranosyl repeating units.
Can Hydrogen ions cross the blood-brain barrier?
Hydrogen ions (H+) cannot cross the blood-brain barrier (BBB; the endothelial cells of the brain capillaries) because they are charged. CO2 on the other hand crosses easily.
What is dextran blue?
Blue Dextran is a high-molecular-weight glucose polymer (original mol wt 2 x 10(6) g/mol) containing covalently bonded Reactive Blue 2 dye (approximately mmol/g dextran). This blue dye is known for its high binding affinity to a wide variety of proteins, with a particularly high affinity for serum albumin.
Why is blue dextran used?
Bearing Cibacron Blue F3G-A, blue dextran is used in affinity chromatography and protein chromatography allowing for the identification of proteins (purified or in crude cellular extracts), and improving the purification procedures for various proteins (1), as well as in gel chromatography as molecular weight markers …
What is the difference between dextran 40 and 70?
Dextrans have high water-binding capacity; for example, 1 g of dextran 40 retains 30 mL of water, while 1 g dextran 70 retains 20–25 mL of water. Dextran is mostly eliminated by the kidneys and should be avoided in patients with impaired renal function.
What is dextran 40 used for?
LMD (dextran 40) is indicated for use in the adjunctive treatment of shock or impending shock due to hemorrhage, burns, surgery or other trauma. It is not indicated as a replacement for whole blood or blood components if they are available.
What is the molecular weight of a dextran?
Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. 8,9,10 Lower MW dextrans will exhibit slightly less branching 4 and have a more narrow range of MW distribution. 11 Dextrans with MW greater than 10,000 behave as if they are highly branched.
What percentage of dextran is bimodal?
At 0.1 M sucrose, pH 5.5, and 28 degrees C, 99.8% of the dextran had a MW>10 (6) Da and at 4.0 M sucrose, 69.9% had a MW<10 (5) Da and 30.1% had a MW>10 (6) Da, giving a bimodal distribution. The degree of branching increased from 5% for 0.1 M sucrose to 16.6% for 4.0 M sucrose.
What is the length of a dextran chain?
Dextran chains are of varying lengths (from 3 to 2000 kilodaltons ). The polymer main chain consists of α-1,6 glycosidic linkages between glucose monomers, with branches from α-1,3 linkages.
How does temperature affect the size of a dextran?
The temperature had very little effect on the size of the dextran, which was >10 (6) Da, but it had a significant effect on the degree of branching, which was 4.8% at 4 degrees C and increased to 14.7% at 45 degrees C.