What are baby hamster kidney cells used for?
18.5. Baby hamster kidney (BHK) cells are currently used for vaccine production as well as production of a few recombinant proteins such as Factor VIIa (NovoSeven®) and FVIII (Kogenate®).
Why are BHK 21 cells used?
The BHK-21 cells are useful for transformations and for stable and temporary transfections. BHK cells are also used to study viral infections.
What are BHK cells used for?
Baby hamster kidney (BHK) cells are most commonly used in the production of animal products, the most important of which is the production of a vaccine against Foot-and-Mouth Disease and rabies [8].
Where do VERO cells come from?
Vero cells are derived from the kidney of an African green monkey, and are one of the more commonly used mammalian continuous cell lines in microbiology, and molecular and cell biology research.
What are HEK 293 cells?
HEK293T cells are a popular derivative of the original HEK293 parent cell line. The HEK293T genome contains the SV40 large T antigen, which enables them to produce recombinant proteins within plasmid vectors containing the SV40 promoter. For this reason, HEK293T is commonly used for retroviral production.
What are C6 36 cells?
C6/36 Aedes albopictus cells are commonly used to study arthropod-borne viruses (arboviruses), such as West Nile virus (WNV).
Do Vero cells make interferon?
Abstract. A clone of Vero cells was isolated and shown to be totally unable to synthesize interferon and insensitive to the toxic effect of poly(rI).
What does Vero cell based mean?
Vero cells are a lineage of cells used in cell cultures. The ‘Vero’ lineage was isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp.; formerly called Cercopithecus aethiops, this group of monkeys has been split into several different species).
Why is it called HEK293?
They are called HEK since they originated in human embryonic kidney cultures, while the number 293 came from Graham’s habit of numbering his experiments; the original HEK 293 cell clone was from his 293rd experiment.
What are 293 cells?
What Is HEK293? HEK293 are immortalized human embryonic kidney cells. Originally isolated in the 1970s by Alex Van der Eb, a Dutch biologist, it was postdoc Frank Graham who transformed the cell line with sheared adenovirus 5 (Ad5). The ‘293’ refers to the fact that it was Graham’s 293rd experiment.
How are HEK293 cells made?
HEK 293 cells were generated in 1973 by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex van der Eb’s laboratory in Leiden, the Netherlands. The cells were obtained from a single, aborted or miscarried fetus, the precise origin of which is unclear.
Baby hamster kidney (BHK) cells are currently used for vaccine production as well as production of a few recombinant proteins such as Factor VIIa (NovoSeven®) and FVIII (Kogenate®).
What causes high titer in baby hamster kidney cells?
Baby Hamster Kidney Cell 1 Arenaviridae*. Arenaviruses replicate noncytolytically to high titer in many kinds of cells, including Vero (African green monkey) and BHK-21 (baby hamster kidney) cells. 2 Arenaviruses. Christopher J. Burrell. 3 Flaviviruses
Are sv3t3 and baby hamster kidney (BHK) cells transformation mutants?
Cell mutants with temperature-sensitive lesions in many properties characteristic of transformed cells have been described for SV3T3 and baby hamster kidney (BHK) cells, similar to the earlier reports of temperature-sensitive transformation mutants of polyoma virus.