How can you distinguish between an artery and vein on a slide?
Veins are closer to the surface of your body, and arteries are deep inside your muscles. The walls of a vein are thinner than an artery.
What are the stains used to demonstrate collagen fibers?
Mason’s Trichrome stain is used to highlight connective tissue fibers in a tissue section. Collagen fibers are stained a blue hue, nuclei a blue-black, and cytoplasm, keratin, and muscle fibers a range of pink to red hues. AZAN Trichrome stain is used to highlight collagen fibers within a tissue section.
What stain is the most commonly used tissue processing?
Haematoxylin and eosin (H & E): Routine stain This is the most common histologic stain, used to differentiate different tissue structures. It also plays an important role in the diagnoses of various pathologies.
What other stains is used for histopathological processing?
Staining techniques used were carmine, silver nitrate, Giemsa, Trichrome Stains, Gram Stain and Hematoxylin among others.
What are Sudan stains used primarily for?
What are Sudan stains used primarily for? Sudan stains are used for staining of lipids and phospholipids. Examples of such stains are sudan black and sudan IV. Oil red O is another stain used for demonstrating fat.
What are the types of stains?
Based on chemical nature: There are three kinds of stain, acidic, basic and neutral, depending upon the chemical nature of the stain. Based on the staining method: There are four kinds of stain, viz. direct, indirect, differential and selective stains.
What is the best preparation done before staining?
Before specific staining can occur, tissue samples must undergo preparation through the following stages: Fixation, processing, embedding, sectioning, and sometimes antigen retrieval. In modern histology laboratories, most of these steps are automated.
What is histochemical staining?
A staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids, and mucins in tissues and fungal hyphae.
What stain do you use to stain the arteries?
Mesentery, H&E, 20X (muscular or medium sized arteries and companion veins, tunica intima, internal elastic lamina, tunica media, tunica adventitia). Aorta , aldehyde fuchsin stain for elastin, 20X (extensive elastin in the wall).
How to differentiate between small and medium arteries histologically?
You do NOT need to worry about distinguishing between small and medium arteries histologically, but you should be aware of the organization of the typical muscular artery, of which these are excellent examples. Note the thin intima, the distinct internal elastic lamina (IEL) and the media composed of smooth muscle cells.
What stain is used on the left ventricle wall?
Slide 102 Heart, left ventricular wall, H&E View Virtual Slide There are 3 stains for this section (H&E, aldehyde fuchsin only, mislabeled Masson, and Masson with hematoxylin counterstain, labeled H & Masson).
How is PAS staining done?
The process of PAS staining usually involves two steps, the first one is the oxidation reaction with periodic acid leading to the formation of aldehydes, the second step is the demonstration of these aldehydes with the help of Schiff’s reagent. Fuchsin dye in Schiff reagent gives a range of colors from magenta to purple.