How do you make a M9 solution?
For 50 ml stock solution dissolve 50 mg biotin in 45 ml water. Add small aliquots of 1N NaOH until the biotin has dissolved. Add water to a final volume of 50 ml. Sterilize the solution over a 0.22-µM filter.
What is M9 minimal?
M9 minimal medium (M9) is commonly used for the cultivation of bacteria (Miller, et al., 1972). It is comprised of a M9 minimal salts base formulation that can be supplemented with various amino acids and carbon sources.
Why M9 medium is used?
Introduction. M9 medium is a minimal growth medium used for bacterial cultures. It has the advantage of being cheap and has a very low autofluorescence (when excited at 488nm) and also very low absorbance.
What is M9 salt?
General description. M9 Minimal Salts is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.
What is the pH of M9 media?
M9 medium with adjusted pH was made according to the M9 recipe. The pH was subsequently increased to pH ~7.4 with the addition of 1 M NaOH. Recipe for M9+ from Cai et al (Cai et al. 2016).
Can you autoclave M9 media?
Filter-sterilize and store at 4°C. Autoclave and store at room temperature.
What is in M9 medium?
M9 minimal medium (standard)
|Reagent||Amount to add (for 100 mL)|
|Glucose (20%; Sigma-Aldrich)a||2 mL|
|MgSO4 (1 M; Fisher Scientific)b||200 μL|
|CaCl2 (1 M; Fisher Scientific)b||10 μL|
How do you make mineral salt agar?
Media and culture condition: Mineral salts medium (MSM) (Zajic and Supplisson, 1972) was prepared by dissolving 1.8 g K2HPO4, 4.0 g NH4 CI, 0.2 g MgSO4. 7H2O, 0.1 g NaCl, 0.01 g FeSO4. 7H2O in 1 L of distilled water. Bacteriological agar was added (15 g L-1) to the solution where solid basal medium is required.
How long does M9 media last?
Hello Maria, Yes Surely you can store M9 medium for long periods (2-3 months). I think to avoid the problem of precipitation you should take care of few things like always use new chemicals and while preparing the medium add the ingredients one by one. Also after complete dissolution of one chemical add the next one.
How do you make mineral salt medium?
What is the use of M9 minimal medium?
M9 minimal medium. This medium is used to grow B. subtilis in the presence of glucose or other variable carbon sources. The medium does contain ammonium and is therefore not suited to change the nitrogen source.
How do you prepare M9 media for chromatography?
Preparation of the medium. For 1 liter of M9 medium add in the following order: 200 mL M9 5x stock (sol. C) 1 mL 100 mM CaCl2 (sol. E) 10 mL trace salts 100x stock (sol.D) 1 mL 1 M MgSO4 (sol.
How do you make a 10x solution of M9 salt?
M9 salt solution (10X) Na2HPO4-2H2O 75.2 g/L KH2PO4 30 g/L NaCl 5 g/L NH4Cl 5 g/L Dissolve the salts in 800 ml water and adjust the pH to 7.2 with NaOH. Add water to a final volume of 1 L and autoclave for 15 min at 121°C. 20% Glucose 20% (w/v) glucose 200 g/L For 500 ml stock solution add 100 g glucose to 440 ml water.