Is BUV395 bright?
BUV395 clearly resolves both dim and abundant populations. In many cases, BUV395 reagents will have brightness similar to or greater than FITC reagents (Table 1). With an excitation max of 348 nm and an emission max of 395 nm, BUV395 can be excited by the 355-nm laser and detected with a 379/28 filter (Figure 1).
Is BUV395 a tandem dye?
355 nm 670/25 BD Horizon Brilliant™ Ultraviolet 661 (BUV661) (Exmax 348 nm/Emmax 661 nm) is a tandem dye that combines BUV395 and an acceptor dye with an emission max at 661 nm.
Is BB700 a tandem dye?
About BB700 Horizon Brilliant™ Blue 700-P (BB700-P) is a tandem dye member of the BD BioSciences Horizon Brilliant Blue family of dyes.
What is PerCP in flow cytometry?
Peridinin Chlorophyll Protein Complex (PerCP)-conjugated antibodies are convenient tools for use in flow cytometry experiments. PerCP is a water soluble carotenoid pigment found in photosynthetic dinoflagellates.
Is BV421 same as Pacific Blue?
For cell surface staining, BV421™ is significantly brighter than other spectrally equivalent fluorophores including Pacific Blue™, BD Horizon™ V450, eFluor® 450, and Alexa Fluor® 405. It has an extinction coefficient of 2,500,000 M-1cm-1 at 405 nm, and an aqueous solution quantum yield of 65 ± 5%.
What channel is the peak channel of the UV laser?
The peak channel (indicated by the black bar) is in channel V11, and it has secondary emission in channels UV11, B7, YG4 and R1. Based on this information, expect this dye to introduce spread into dyes emitting at similar wavelengths. Ultraviolet (16 channels) Dyes Primarily Excited by the Ultraviolet Laser 4
Which laser channels are excited by this dye?
This dye is excited by all 5 lasers. The peak channel (indicated by the black bar) is in channel V11, and it has secondary emission in channels UV11, B7, YG4 and R1. Based on this information, expect this dye to introduce spread into dyes emitting at similar wavelengths.
What are the best fluorochrome selection guidelines for 5 laser systems?
Fluorochrome Selection Guidelines for 5 Laser Systems (UV-V-B-YG-R) Last Updated: March 2019 Fluorochrome Signatures Dyes can be used in combination as long as they have a unique spectrum signature. When designing a multicolor panel, you should look for dyes with unique spectra and also consider spread introduced by the dyes (see slide 28). 2
What makes spectra precision laser products so popular?
Spectra Precision Laser products are well known for their technology, quality, ease of use, and ruggedness. In addition, Spectra Precision Laser products benefit from strong support through the world’s largest network of repair centers. All of this makes Spectra Precision Laser the brand of choice for construction site prep and surveying tools.