What are GAL4 lines?
The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs to manipulate neuronal function.
What is GAL4 biology?
The GAL4-UAS system is a biochemical method used to study gene expression and function in organisms such as the fruit fly. It is based on the finding by Hitoshi Kakidani and Mark Ptashne, and Nicholas Webster and Pierre Chambon in 1988 that Gal4 binding to UAS sequences activates gene expression.
How does GAL4 work?
Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in DNA. It then recruits transcription machinery to the site to induce gene expression. Thus, genes and siRNA encoded downstream of the UAS sequence are only expressed when Gal4 is expressed.
What chromosome is GAL4 on?
| Line | Gene | Chr. |
|---|---|---|
| Line | Gene | Chr. |
| CR00323 | Marf | X |
| CR00446 | Dsor1 | X |
Who discovered GAL4?
11 Method 2: The Drosophila UAS/GAL4 Expression System. The standard expression system used in Drosophila is the bipartite GAL4–UAS system first described by Brand and Perrimon (1993).
Does GAL4 bind to DNA?
Abstract. The DNA binding domain of the GAL4 transcription factor from yeast is located in the N-terminal 60 residues of the polypeptide of 881 amino acids.
Is GAL4 an activator?
Yeast Gal4, one of the first known acidic activators, is responsible for the regulation of genes involved in galactose catabolism (41).
Who discovered Gal4?
How many domains does GAL4?
GAL4 has two domains whose activities can be separated: the DNA-Binding domain and the transcriptional Activation Domain.
