What does fluorescence recovery after photobleaching show about the membrane structure?
Fluorescence recovery after photobleaching (FRAP) is a method for determining the kinetics of diffusion through tissue or cells. It is capable of quantifying the two dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes, or to examine single cells.
For what purpose is the biological laboratory technique of fluorescence recovery after photobleaching FRAP used?
FRAP (Fluorescence recovery after photobleaching) is used to characterize the mobility of cellular molecules.
What is the bleached area in a FRAP fluorescence recovery after photobleaching experiment?
Thanks to the development of GFP fusion proteins, a revival of FRAP applications was seen in the mid-1990s. In FRAP, fluorescent molecules in a region of interest (ROI) within the plasma membrane are bleached by a high-intensity laser source.
Which membrane would show a more rapid recovery of fluorescence in a FRAP study?
A. The FRAP curve for lipids would show a much more rapid recovery to initial levels of fluorescence.
What does FRAP stand for?
FRAP
| Acronym | Definition |
|---|---|
| FRAP | Fluorescence Recovery After Photobleaching |
| FRAP | Fire and Resource Assessment Program (California) |
| FRAP | Fédération des Radios Associatives en Pays de la Loire (French radio association) |
| FRAP | Federal Rules of Appellate Procedure |
What is fluorescence photobleaching?
Photobleaching (also termed fading) occurs when a fluorophore permanently loses the ability to fluoresce due to photon-induced chemical damage and covalent modification.
Which membrane would show a more rapid recovery of fluorescence in a FRAP study quizlet?
A membrane containing a larger proportion of saturated fatty acids would show a more rapid recovery in a FRAP study.
Which is the most abundant phospholipid in animal cell membranes?
Lecithin (q.v.; phosphatidyl choline) and the cephalins (phosphatidyl ethanolamine and phosphatidyl serine) are groups of phospholipids of widespread occurrence in plants and animals; lecithin is the most abundant, but is rare in microorganisms.
What is FRAP and FLIP?
The major difference between these two microscopy techniques is that FRAP involves the study of a cell’s ability to recover after a single photobleaching event whereas FLIP involves the study of how the loss of fluorescence spreads throughout the cell after multiple photobleaching events.
What is fluorescence recovery after photobleaching?
Fluorescence recovery after photobleaching. Fluorescence recovery after photobleaching (FRAP) is a method for determining the kinetics of diffusion through tissue or cells. It is capable of quantifying the two dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes, or to examine single cells.
What is the ratio FA/F0 after photobleaching?
It then obtains the ratio Fa / F0, in which Fa is the first slice after photobleaching and F0 is the slice just before photobleaching. Even with low illumination during the recovery phase, fluorescence loss through photobleaching is expected to occur over the course of the recovery curve.
Does diffusion along the z axis contribute to fluorescence recovery?
Then diffusion along the z axis does not cause fluorescence recovery as all protein is bleached uniformly along the z axis, and so neglecting it, as Soumpasis’ equation does, is harmless. However, if diffusion along the z axis does contribute to fluorescence recovery then it must be accounted for.
Can FRAP be used to monitor protein fluorescence recovery?
This observation has most recently been exploited to investigate protein binding. Similarly, if the GFP labeled protein is constitutively incorporated into a larger complex, the dynamics of fluorescence recovery will be characterized by the diffusion of the larger complex. FRAP can also be used to monitor proteins outside the membrane.