What is C18 2 column?

What is C18 2 column?

Luna® C18(2) is a reversed phase column that provides a measurable improvement over many HPLC columns for two important chromatographic properties – resolution and peak shape. This HPLC column has a fully porous silica as solid support. Recommended for separation of hydrophobic compounds.

What are Luna columns?

Luna C18(2) phase is an octadecyl silane with ligands bound to the silica surface,resulting in a C18 HPLC column with very hydrophobic stationary phase that offers great methylene selectivity. Phase.

What is C18 column?

C18 columns are HPLC (high performance liquid chromatography) columns that use a C18 substance as the stationary phase. C18 HPLC columns are used in environmental sciences and chemical analysis, as well as industries such as pharmaceutical and environmental sciences, to analyze individual parts of chemical mixtures.

What is TMS Endcapping?

In chromatography, endcapping refers to the replacement of accessible silanol groups in a bonded stationary phase by trimethylsilyl groups. End-capped columns have much lower residual silanol group activity compared to non-endcapped columns.

What is Agilent column?

Small Molecule Columns Agilent offers one of the broadest, most modern liquid chromatography (LC) column portfolios for small molecule analysis, with columns suitable for nearly all separation modes and applications.

What is the HPLC column?

HPLC is a highly improved form of column chromatography. A pump forces a solvent through a column under high pressures of up to 400 atmospheres. The column packing material or adsorbent or stationary phase is typically a granular material made of solid particles such as silica or polymers.

Why we use C18 column in HPLC?

C18 is considered better for separating compounds such as long-chain fatty acids as compared to relatively small organic compounds. It is relatively cheap as it is produced in large amounts by most manufacturers.

What is Luna C18 (2) phase?

Luna C18 (2) phase is an octadecyl silane with ligands bound to the silica surface,resulting in a C18 HPLC column with very hydrophobic stationary phase that offers great methylene selectivity C18 (2) Ultra pure, metal-free silica (99.99% purity) (USP:L1) Ultra-pure, metal-free silica (99.99 % purity) is the back bone of all Luna material.

What is a polar end-capped C18 phase?

The polar end-capped C18 phase provides optimal retention of polar analytes and is fully compatible with 100% aqueous mobile phases. Manufactured and rigorously tested…

What is C18 phase in HPLC?

Luna C18 (2) phase is an octadecyl silane with ligands bound to the silica surface,resulting in a C18 HPLC column with very hydrophobic stationary phase that offers great methylene selectivity C18 (2) Ultra pure, metal-free silica (99.99% purity) (USP:L1)

What are the characteristics of C18 L1 ligand?

Stationary Phase Category: C18, octadecylsilane (L1) Ligand Type: End-capped C18 Particle: 1.8 μm, 3 μm, or 5 μm fully porous silica Pore Size: 100 Å Carbon Load: 20% End-Cap: yes Surface Area: 300 m2/g pH Range: 2.0–8.0 Maximum…