What is IBMX and how does it work?
IBMX is an inhibitor of cyclic nucleotide phosphodiesterases (PDEs; IC₅₀ = 19, 50, 18, 13, 32, 7, and 50 µM for PDE1, PDE2, PDE3, PDE4, PDE5, PDE7, and PDE11, respectively). By inhibiting PDEs, IBMX increases cellular cAMP and cGMP levels, activating cyclic-nucleotide-regulated protein kinases.
What is PDE enzyme?
Phosphodiesterases (PDEs) are enzymes involved in the homeostasis of both cAMP and cGMP. They are members of a family of proteins that includes 11 subfamilies with different substrate specificities. Their main function is to catalyze the hydrolysis of cAMP, cGMP, or both.
What does Ibmx stand for?
How do you dissolve Ibmx?
A stock solution may be made by dissolving the IBMX in the solvent of choice, which should be purged with an inert gas. IBMX is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of IBMX in ethanol and DMX is approximately 5 mg/ml and approximately 10 mg/ml in DMSO. 1.
What is cell PDE?
Phosphodiesterases (PDEs) are enzymes that regulate the intracellular levels of cyclic adenosine monophosphate and cyclic guanosine monophosphate, and, consequently, exhibit a central role in multiple cellular functions.
What is PDE biology?
Cyclic nucleotide phosphodiesterases (PDEs) are important drug targets with unique tissue distribution and functional properties. This family of enzymes is key in the regulation of cyclic nucleotide second messenger levels because they degrade both cAMP and cGMP.
How does forskolin increase cAMP?
Forskolin increases intracellular cAMP level through activation of adenylyl cyclase in various cell types. In this study, the authors find that forskolin-induced increases in cAMP levels enhance mAb production in recombinant CHO cell cultures.
How do you dilute dexamethasone for cell culture?
Once dexamethasone is solubilized, dilutions can be prepared by adding sterile culture medium. To obtain a solution at 20 μg/ml (51 μM), add 49 ml of culture medium to 1 ml of the stock solution. Aqueous solutions should not be stored for more than one day.
What is PDE in biology?
What is the PDE protein?
Phosphodiesterases (PDEs) are enzymes that regulate the intracellular levels of cAMP and cGMP. Protein kinase A or cAMP-dependent protein kinase mediates most cAMP effects in the cell. Over the last 25 years, various components of this group of molecules have been involved in human diseases, both genetic and acquired.
What is the meaning of PDE?
Partial Differential Equation (abbreviated in the following as PDE in both singular and plural usage) is an equation for an unknown function of two or more independent variables that involves partial derivatives.