What is Nunc plate?

What is Nunc plate?

Obtain consistent and reliable results with Thermo Scientific™ Nunc™ MicroWell™ 96-Well Microplates. With multiple surface modifications, these plates are ideal for cell-based assays as well as biochemical assays. The flat bottom shape is suitable for microscopic and optical measurements.

Why do plates have 96 wells?

It also maintains a 2:3 matrix on the plates, which allows to easily run your samples in duplicate or triplicates. So if you scale up from 96 to 384 you have the same ratio which is easier to work with when you look at it and arrange your wells. 96 is probably the one that was used the most so it’s now the popular one.

What are MaxiSorp plates?

Immuno plates, MaxiSorp surface—These plates are designed for solid phase immuno-assays and have a polystyrene surface with high affinity for polar groups and hydrophilic molecules.

What is a 96-well plate?

96-well plates are designed for High Throughput Screening (HTS), sample storage, cell culture, and DNA extraction involving a large sample size. This product has strong durability, with high temperature and chemical resistance.

What is nunclon Delta surface?

Nunclon Delta is a cell culture–treated surface modification that makes the polystyrene surface of your culture vessel more hydrophilic, promoting maximum adhesion for a broad range of cell types. This tissue-culture (TC) surface is offered across multiple formats for your adherent cell culture applications.

Are all 96 well plates the same size?

Corning 96-well polystyrene microplates have plate dimensions (length x width x height) of 127.76 x 85.48 x 14.22 mm that meet standard ANSI/SBS footprint dimensions for microplates. Corning tissue culture-treated microplates have the same surface treatment used on other Corning culture vessels.

What is a 24-well plate used for?

AcroPrep 24-well filter plates with Omega membrane can be used for ultrafiltration applications including desalting, diafiltration, concentration or fractionation of protein and nucleic acid samples.

How do you coat plate on Elisa?

Coating of Capture Antibody

  1. Appropriately dilute the capture or coating antibody in carbonate-bicarbonate buffer or PBS.
  2. Pipette 0.2 ml of the diluted capture antibody to each well of a microtiter plate.
  3. Incubate the plate (covered) for 1 hour at 37 °C.
  4. Remove the coating solution.

How many UL can a 96 well plate hold?

CulturPlate Microplates

Well format 24-well 96-well
#columns 6 12
Well volume 2.39 mL 400 µL
Recommended working volume 0.5-2.39 mL 80 µL-350 µL
Height (mm) 18.70 14.60

How do you plate cells in a 96 well plate?

Cell doubling time is an important factor to be considered when adjusting cell density at the beginning of an experiment. Plate 200 µL of cell culture (i.e., 10,000–50,000 cells) into the wells of the sterile 96-well cell culture plate. Incubate the cells for 18 hours at 37°C. Stimulate the cells as desired.

What is nunclon Delta treatment?

What does TC treated mean?

“Tissue-culture treated” is for cell culture of anchorage-dependent cells and “non-treated” is for suspension cell culture applications, such as mammalian cells that grow in suspension and bacterial cell culture where attachment is not required. Cite.

What are the features of the Nunc-immuno 96 microwell solid plates?

Nunc-Immuno 96 MicroWell solid plates are intended for quantitative and qualitative solid phase immuno assays (i.e. ELISA) and binding assays. Raised rims on the wells Certified reproducibility of binding

What is a 96 well plate made of?

Thermo Scientific™ Nunc MicroWell 96-Well Optical-Bottom Plates with Polymer Base have a solid polystyrene black or white upper structures with a polystyrene film at the bottom of the plate. The plates are optimized for fluorescence and luminescence in cell culture and microscopic applications.

What is a nunc 96 filter plate?

Efficiently perform purification of high quality plasmid DNA or PCR products with the Thermo Scientific™ Nunc™ 96 Deep Well Filter Plates. Made of chemical resistant polypropylene material with superior mechanical strength, these plates conform to standard 96 well footprint compatible with high throughput sample processing.

What is the maxisorp™ high protein-binding capacity 96 well ELISA plate?

The Nunc MaxiSorp™ high protein-binding capacity 96 well ELISA plates are recommended for use in capturing immunoglobulins found in eBioscience Ready-SET-Go! ELISA kits, as well as other matched antibody pairs for ELISA. Maxisorp plates have a protein-binding capacity of approximately 600-650 ng IgG/cm 2.