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What is RNA polymerase II used for?

Table of Contents

  • What is RNA polymerase II used for?
  • What does RNA pol 2 produce?
  • What is Pol II transcribe?
  • What does RNA pol III do?
  • How many polymerases are there?
  • How does RNA polymerase II terminate?

What is RNA polymerase II used for?

RNA polymerase II catalyzes the synthesis of precursor mRNA. In eukaryotes, this RNA is generally longer than the final or “mature” mRNA, whose molecule is used as a template for protein synthesis.

What does RNA pol 2 produce?

RNA polymerase II (pol II) is a DNA-dependent RNA polymerase that is responsible for transcription of protein-coding genes in the nucleus of eukaryotic cells. Pol II transcription results in synthesis of an RNA copy of the protein-coding DNA strand of genes.

What is the difference between RNA Pol I and Pol III?

RNA polymerase I (RNAPI) transcribes rRNA genes, RNA polymerase II (RNAPII) transcribes mRNA, miRNA, snRNA, and snoRNA genes, and RNA polymerase III (RNAPIII) transcribes tRNA and 5S rRNA genes. This is in contrast with prokaryotes where a single RNA polymerase is responsible for the transcription of all genes.

What are pol II promoters?

The RNA polymerase II core promoter is generally defined to be the sequence that directs the initiation of transcription. This simple definition belies a diverse and complex transcriptional module. There are two major types of core promoters – focused and dispersed.

What is Pol II transcribe?

RNA polymerase II (RNAP II and Pol II) is a multiprotein complex that transcribes DNA into precursors of messenger RNA (mRNA) and most small nuclear RNA (snRNA) and microRNA. It is one of the three RNAP enzymes found in the nucleus of eukaryotic cells.

What does RNA pol III do?

RNA polymerase III (Pol III) transcribes various small stable RNAs that are essential in multiple cellular pathways, including pre-mRNA splicing (U6 snRNA) and protein synthesis (5S rRNA, tRNAs)2.

What does RNA Pol II transcribe?

RNA polymerase II (Pol II) transcribes all protein-coding genes and many noncoding RNAs in eukaryotic genomes. Although Pol II is a complex, 12-subunit enzyme, it lacks the ability to initiate transcription and cannot consistently transcribe through long DNA sequences.

What does RNA pol II transcribe?

How many polymerases are there?

Cells contain at least 13 documented DNA polymerases that might have different functions. The five earliest-identified DNA polymerases (α, β, δ, ε and γ) are essential proteins that have key roles in nuclear DNA- or mitochondrial DNA-mediated transactions.

How does RNA polymerase II terminate?

Pol II escapes from the promoter when RNA reaches a length of ∼8–9 nucleotides, which constitutes the full length of the DNA–RNA hybrid that is observed during the elongation stage. Termination occurs when Pol II ceases RNA synthesis and both Pol II and the nascent RNA are released from the DNA template.

Does RNA Pol II require transcription factors?

A minimal RNA polymerase II (pol II) transcription system comprises the polymerase and five general transcription factors (GTFs) TFIIB, -D, -E, -F, and -H. The addition of Mediator enables a response to regulatory factors. The GTFs are required for promoter recognition and the initiation of transcription.

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