What is SMARTpool siRNA?
The SMARTpool (available for ON-TARGETplus, Accell, siGENOME and Lincode siRNA) combines four gene-specific siRNAs into a single reagent pool. Research has shown that pooled siRNAs: Improve the likelihood of effective gene silencing.
How does siRNA bind to mRNA?
Once the siRNA is part of the RISC complex, the siRNA is unwound to form single stranded siRNA. Once the single stranded siRNA (part of the RISC complex) binds to its target mRNA, it induces mRNA cleavage. The mRNA is now cut and recognized as abnormal by the cell.
What is the protocol for reverse transfection?
Protocol – Reverse Transfection. Use this procedure to reverse transfect Stealth RNAi or siRNA into mammalian cells in a 24-well format (for other formats, see Scaling Up or Down Transfections). In reverse transfections, the complexes are prepared inside the wells, after which cells and medium are added.
How do I reverse transfect stealth RNAi into mammalian cells?
Use 10 nM RNAi duplex and indicated procedure as a starting point; optimize transfections as described in Optimizing Transfections. Use this procedure to reverse transfect Stealth RNAi or siRNA into mammalian cells in a 24-well format (for other formats, see Scaling Up or Down Transfections).
How do I perform a reverse transfection with Lipofectamine rnaimax?
If you want to use Lipofectamine RNAiMAX for your cotransfections, perform a reverse transfection with the following modifications: 1: Add 20 ng (for 24-well format) of plasmid DNA to the diluted RNAi duplex.
What is the role of co-transfection for small RNA?
(C) Co-transfecting a small RNA molecule and a plasmid DNA that carries a reporter system can be used to assess small RNA transfection efficiency or to determine the regulatory effects of the small RNA on a specific gene. Generally, multiple plasmid DNA co-transfection aims to introduce more than one type of foreign genes into the host cells.