What is the numerical aperture of the low power objective?
Numerical aperture is generally the most important design criteria (other than magnification) to consider when selecting a microscope objective. Values range from 0.1 for very low magnification objectives (1x to 4x) to as much as 1.6 for high-performance objectives utilizing specialized immersion oils.
What is the numerical aperture of 40x?
Table 1 – Numerical Aperture versus Optical Correction
| Magnification | Plan Achromat (NA) | Plan Fluorite (NA) |
|---|---|---|
| 4x | 0.10 | 0.13 |
| 10x | 0.25 | 0.30 |
| 20x | 0.40 | 0.50 |
| 40x | 0.65 | 0.75 |
What is the numerical aperture of each objective?
The numerical aperture of a microscope objective is the measure of its ability to gather light and to resolve fine specimen detail while working at a fixed object (or specimen) distance.
What is low power objective on a microscope?
Low power objectives cover a wide field of view and they are useful for examining large specimens or surveying many smaller specimens. This objective is useful for aligning the microscope. The power for the low objective is 10X. Place one of the prepared slides onto the stage of your microscope.
What is the numerical aperture of 4x objective lens?
The MVC-4X microscope objective has a 4x magnification, 0.1 numerical aperture, and 15.1 mm working distance. It uses a standard RMS thread, 0.800-36. The lenses are antireflection coated for the visible spectrum. This MVC-4x objective is suitable for use with standard low power visible laser wavelengths.
How does numerical aperture affect resolution?
Numerical aperture determines the resolving power of an objective, but the total resolution of the entire microscope optical train is also dependent upon the numerical aperture of the substage condenser. The higher the numerical aperture of the total system, the better the resolution.
What is numerical aperture?
Numerical aperture is a measure of the highly diffracted light rays captured by the objective. In practice, it is difficult to achieve numerical aperture values above 0.95 with dry objectives. Figure 1 illustrates a series of light cones derived from objectives of varying focal length and numerical aperture.
What is the numerical aperture equation of a microscope?
In the numerical aperture equation, n represents the refractive index of the medium between the objective front lens and the specimen, and µ or α is the one-half angular aperture of the objective. The numerical aperture of a microscope objective is a measure of its ability to gather light and resolve fine specimen detail at a fixed object distance.
What is the numerical aperture of the 100X objective?
The 100x objective lens is also rated at 1.25. The medium between the 100x lens and the slide can be air or oil. Without getting too technical, the only way to get a Numerical Aperture greater than 1.0 is to use a material with a refractive index greater than 1.0.
How to specify the aperture size of an objective?
In photography, it is not common to specify the numerical aperture of an objective, because such objectives are not thought to be used with a fixed working distance. Instead, one often specifies the aperture size with the so-called f-number, which is the focal length divided by the diameter of the entrance pupil .