What is the principle of Jaffe reaction?

Creatinine in serum or urine is determined by Jaffe’s reaction where creatinine produces quantitatively an orange color with picric acid in alkaline medium. After allowing an incubation time of 15 min at room temperature for color development the color is measured at 520 nm.

What is the Jaffe method?

The Jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine.

What interferes with the Jaffe reaction?

The Jaffe reaction is not specific for creatinine, the same reaction resulting in Jaffe-like chromogens show many interfering substances (ascorbic acid, bilirubin, proteins, ketones, cephalosporins and other drugs).

How do you calculate serum creatinine?

eCcr by wet chemistry=(140-age) × b.w (kg) × 0.85 for females ÷ 72 × serum creatinine (mg/dl by wet chemistry) = (140-60) × 65 × 0.85 ÷ 72 × 2.1=4420 ÷ 151.2=29.23 ml/min. It is evident from the example considered that the values of serum creatinine are inversely related to the estimated clearance of creatinine.

What is creatinine enzymatic test?

Enzymatic Creatinine Labtest uses the enzymes creatinine. aminohydrolase, creatine amidinohydrolase, and sarcosine oxidase coupled with the Trinder reaction to determine the creatinine concentration in serum, plasma, and urine samples.

What’s normal urine creatinine?

Normal urine creatinine values generally range from 955 to 2,936 milligrams (mg) per 24 hours for males, and 601 to 1,689 mg per 24 hours for females, according to the Mayo Clinic. Creatinine values that fall outside the normal range may be an indication of: kidney disease.

What is spot urine creatinine?

Creatinine – Spot is the test to measure the amount of creatinine in urine. Creatinine is the waste product of muscles metabolism. Normally creatinine is excreted out in urine but detection of abnormal levels (both high and low) of creatinine in urine may be due to impaired filteration capacity of kidneys.

What units is creatinine measured in?

In the United States and in most European countries creatinine is usually reported in m g/d L, whereas in Canada, Australia, and a few European countries, μ mol/L is the usual unit. One mg/dL of creatinine is 88.4 μmol/L.

What is assay creatinine?

A creatinine test is a measure of how well your kidneys are performing their job of filtering waste from your blood. Creatinine is a chemical compound left over from energy-producing processes in your muscles.

What is the Jaffe reaction?

Jump to navigation Jump to search. The Jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine.

What is the Jaffe reaction used to measure creatinine?

Jaffe reaction. The Jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine.

What is the Jaffe reaction of creatinine and picric acid?

Jaffe reaction of creatinine and picric acid —the oldest clinical methodology still in use. The Jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine.