What was the role of guaiacol in the enzymatic reaction?

If the enzyme is present, the peroxide is quickly converted to water and oxygen, and the oxygen reacts with the Guaiacol to produce a brown product, oxidized Guaiacol. The brown color can be measured in a spectrophotometer.

What does turnip peroxidase do?

Peroxidases use a reducing agent to catalyze the decomposition of hydrogen peroxide. Any cell using molecular oxygen in its metabolism will produce small amounts of H2O2 as a highly toxic byproduct.

How do peroxidase enzymes work?

Peroxidase are enzymes that catalyze oxidation-reduction reaction by mechanism of free radical that transform several compounds into oxidized or polymerized products. The prosthetic group of peroxidase is composed of a protein-bound heme, usually through a histidine residue that acts as a proximal ligand.

Is guaiacol a reducing agent?

Many endogenous organic compounds may be used as reducing agents in Equation 2. One of the most common and convenient reducing agents for this purpose is guaiacol, a colourless compound having the formula C7H8O2.

What is guaiacol used for with peroxidase?

Guaiacol peroxidases are heme-containing proteins that preferably oxidize aromatic electron donors such as guaiacol and pyragallol at the expense of H2O2 (Sharma et al. 2012). Guaiacol peroxidase is associated with many important biosynthetic processes and defense against abiotic and biotic stresses.

What is guaiacol peroxidase?

Is guaiacol a substrate or product?

Determination of enzymatic characteristic properties such as Michaelis–Menten constant (Km), temperature optima and pH optima using different phenolic compounds, indicated p-cresol as a potential substrate for the peroxidase enzyme assay at room temperature, whereas guaiacol, which is widely used as a substrate for …

Is guaiacol a substrate?

Guaiacol was used as the target phenolic compound because it is a widely known substrate used for assaying peroxidase activity in the extract from different sources.

What is the importance of peroxidase?

Peroxidase is an enzyme found in a wide variety of organisms, from plants to humans to bacteria. Its function is to break down hydrogen peroxide (H2O2), which is one of the toxins produced as a byproduct of using oxygen for respiration. (The fact that it’s toxic is what makes hydrogen peroxide useful in first aid kits.

What does guaiacol mean?

guaiacol. / (ˈɡwaɪəˌkɒl) / noun. a yellowish oily creosote-like liquid extracted from guaiacum resin and hardwood tar, used medicinally as an expectorant.

Guaiacol peroxidases are heme-containing proteins that preferably oxidize aromatic electron donors such as guaiacol and pyragallol at the expense of H2O2(Sharma et al. 2012). Guaiacol peroxidase is associated with many important biosynthetic processes and defense against abiotic and biotic stresses.

What is the role of guaiacol in cyclooxygenase reactions?

Guaiacol is readily oxidized by the heme iron of peroxidases including the peroxidase of cyclooxygenase (COX) enzymes. It therefore serves as a reducing co-substrate for COX reactions. Guaiacol is a phenolic natural product first isolated from Guaiac resin and the oxidation of lignin.

How do you make guaiacol activity staining solution?

For the activity staining solution, 10 mL of solution A [100 µL guaiacol 25% (v/v) dissolved in 15 mL ethanol, completed to 100 mL with 50 mM phosphate buffer pH 7] was mixed with 9 mL 50 mM phosphate buffer pH 7. Then 1 mL 0.3% hydrogen peroxide was added.

What is guaiacol?

Guaiacol is a phenolic natural product first isolated from Guaiac resin and the oxidation of lignin. Guaiacol is also present in wood smoke, as a product of pyrolysis of lignin.